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Recombinant fragment corresponding to Chicken Actin aa 50-70 (N terminal). The immuogen is purified chicken gizzard actin. This is predominantly smooth muscle gamma-actin. Is a pan-actin which recognizes all actin isoforms.
Our Abpromise guarantee covers the use of ab14128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 20 µg/ml. Predicted molecular weight: 43 kDa.
On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43kDa protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha actin found in skeletal, cardiac and arterial muscle.
Methanol fixed HeLa and NIH/3T3 cells
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab14128 overnight at 4°C. Antibody binding was detected using ab175783 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
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