Overview
- Product nameAnti-Actin antibody [2Q1055]See all Actin primary antibodies ...
- DescriptionMouse monoclonal [2Q1055] to Actin
- Specificityab14128 recongizes all six isoforms of vertebrate actin, globular (G) and fillimentous (F) forms, it is a pan-actin antibody that binds to an epitope in a highly conserved region of actin. ab14128 labels myotubes and stains myoblasts and fibroblasts, it does not interfer with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. In ELISA, ab14128 shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins, the antibody also shows significant binding to both Dictyostelium discoidum and Physarum polycephalum in ELISA.
- Tested applicationsELISA, WB, ICC, IHC-P more details
- Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Donkey, Goat, Chicken, Guinea pig, Hamster, Cow, Cat, Dog, Human, Pig, Saccharomyces cerevisiae, Tetrahymena sp., Bird, Caenorhabditis elegans, Fruit fly (Drosophila melanogaster), Monkey, a wide range of other species, all Mammals, Escherichia coli , Goose, Macaque Monkey, Frog, Equus, Rooster
- Immunogen
Chicken gizzard actin
- EpitopeThe epitope recognized by the antibody appears to be located in the N-terminal two thirds of the actin molecule, possibly near amino acids 50-70.
- General notesFor maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: 40% Glycerol, 10mM PBS, pH 7.6 -
Concentration information loading... - PurityProtein A purified
- Clonality Monoclonal
- Clone number2Q1055
- IsotypeIgG1
- Light chain typekappa
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab14128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: Use at an assay dependent dilution. In ELISA, shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows significant binding to both Dictyostelium discoidum and Physarum polycephalum in ELISA. |
| WB | WB: Use a concentration of 1 - 20 µg/ml. Predicted molecular weight: 43 kDa. On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha actin found in skeletal, cardiac and arterial muscle. |
| ICC | ICC: 1/500. Methanol fixed mouse 3T3 cells. |
| IHC-P | IHC-P: Use a concentration of 10 µg/ml. |
Target
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions. - Sequence similaritiesBelongs to the actin family.
- Cellular localizationCytoplasm > cytoskeleton.
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Database links
- Entrez Gene: 58 Human
- Entrez Gene: 11459 Mouse
- Entrez Gene: 29437 Rat
- Omim: 102610 Human
- SwissProt: P68133 Human
- SwissProt: P68134 Mouse
- SwissProt: P68136 Rat
- Unigene: 1288 Human
- Unigene: 214950 Mouse
- Unigene: 82732 Rat
see all
Target information above from: UniProt accession
P68133
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ACTA antibodyACTA1 antibodyActin alpha skeletal muscle antibody
- Actin antibodyactin, alpha 1, skeletal muscle 1 antibodyactin, alpha 1, skeletal muscle antibodyActin, alpha skeletal muscle antibodyACTS_HUMAN antibodyalpha Actin 1 antibodyalpha skeletal muscle Actin antibodyalpha skeletal muscle antibodyAlpha-actin-1 antibodyASMA antibodyCFTD antibodyCFTD1 antibodyCFTDM antibodyMPFD antibodyNEM1 antibodyNEM2 antibodyNEM3 antibodynemaline myopathy type 3 antibody
see all
References for Anti-Actin antibody [2Q1055] (ab14128)
This product has been referenced in:
- Siegel MP et al. Reduced coupling of oxidative phosphorylation in vivo precedes electron transport chain defects due to mild oxidative stress in mice. PLoS One 6:e26963 (2011). WB ; Mouse . Read more (PubMed: 22132085) »
- Millson SH et al. A simple yeast-based system for analyzing inhibitor resistance in the human cancer drug targets Hsp90alpha/beta. Biochem Pharmacol 79:1581-8 (2010). WB ; Saccharomyces cerevisiae . Read more (PubMed: 20138026) »
