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Products:Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
Overview
Product name
Anti-Actin antibody [2Q1055]
See all Actin products (22) ...
Description
Mouse monoclonal [2Q1055] to Actin
Specificity
ab14128 recongizes all six isoforms of vertebrate actin, globular (G) and fillimentous (F) forms, it is a pan-actin antibody that binds to an epitope in a highly conserved region of actin. ab14128 labels myotubes and stains myoblasts and fibroblasts, it does not interfer with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. In ELISA, ab14128 shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins, the antibody also shows significant binding to both Dictyostelium discoidum and Physarum polycephalum in ELISA.
Tested applications
ELISA, WB, ICC, IHC-Pmore details
Cross reactivity
Reacts with a wide range of species. Species specifically tested: Avian, Bovine, Burro, C. elegans, Canine, Chicken, Donkey, Drospohila, E. coli, Equine, Feline, Frog, Goat, Goose, Guinea Pig, Hamster, Human, Macaque, Mammal, Monkey (Rhesus), Mouse, Porcine, Rabbit, Rat, Rodent, Rooster, Sheep, Tetrahymena, Xenopus and Yeast.
Immunogen
Chicken gizzard actin
Epitope
The epitope recognized by the antibody appears to be located in the N-terminal two thirds of the actin molecule, possibly near amino acids 50-70.
General notes
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 40% Glycerol, 10mM PBS, pH 7.6
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
2Q1055
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> RIP
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Crosslinking
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Tropomyosin
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Troponin
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution. (In ELISA, shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows significant binding to both Dictyostelium discoidum and Physarum polycephalum in ELISA.)
WB: Use a concentration of 1 - 20 µg/ml.Predicted molecular weight: 43 kDa.(On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha actin found in skeletal, cardiac and arterial muscle.)
ICC: 1/500(Methanol fixed mouse 3T3 cells.)
IHC-P: Use a concentration of 10 µg/ml
Target
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in disease
Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
Sequence similarities
Belongs to the actin family.
Cellular localization
Cytoplasm > cytoskeleton.
Target information above from: UniProt accessionP68133
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- alpha skeletal muscle antibody
- ACTA antibody
- ACTA1 antibody
see all
References for Anti-Actin antibody [2Q1055] (ab14128)
This product has been referenced in:
- Siegel MPet al. Reduced coupling of oxidative phosphorylation in vivo precedes electron transport chain defects due to mild oxidative stress in mice. PLoS One 6:e26963 (2011). WB; Mouse.Read more (PubMed: 22132085) »
- Millson SHet al. A simple yeast-based system for analyzing inhibitor resistance in the human cancer drug targets Hsp90alpha/beta. Biochem Pharmacol 79:1581-8 (2010). WB; Saccharomyces cerevisiae.Read more (PubMed: 20138026) »
See all 5 publications for this product
Publishing research using ab14128? Please let us know so that we can cite the reference in this datasheet
![Actin antibody [2Q1055] for ELISA in Human (14128)](/ps/Reviews/Images/ab13176_78032.jpg)
![Actin antibody [2Q1055] for WB in Mouse (14128)](/ps/Reviews/Images/ab13238_75650.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"