Anti-Actin antibody [3F10] (ab18146)
- Product nameAnti-Actin antibody [3F10]See all Actin primary antibodies ...
- DescriptionMouse monoclonal [3F10] to Actin
- SpecificityReacts with alpha- smooth muscle as well as alpha-skeletal and alpha-cardiac (sarcomeric) isoform of actin. Reacts with tumors arising from smooth muscle (leiomyosarcomas) as well as skeletal muscle (rhabdomyosarcomas).
- Tested applicationsIHC-P, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Horse, Chicken, Dog, Human, Pig
Predicted to work with: Silk worm
Full length purified Actin (Human).
- Positive controlMuscle or sarcoma
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 40% Glycerol, 0.2% BSA, 10mM PBS, pH 7.4
- Concentration information loading...
- PurityProtein G purified
- Purification notesProtein G affinity purified.
- Clonality Monoclonal
- Clone number3F10
- Light chain typekappa
Our Abpromise guarantee covers the use of ab18146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 1 - 2 µg/ml for 30 min at RT. No special pretreatment is required for immunohistochemical staining of formalin-fixed tissues.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
- Sequence similaritiesBelongs to the actin family.
- Cellular localizationCytoplasm > cytoskeleton.
- ACTA antibodyACTA1 antibodyActin alpha skeletal muscle antibody
- Actin antibodyactin, alpha 1, skeletal muscle 1 antibodyactin, alpha 1, skeletal muscle antibodyActin, alpha skeletal muscle antibodyACTS_HUMAN antibodyalpha Actin 1 antibodyalpha skeletal muscle Actin antibodyalpha skeletal muscle antibodyAlpha-actin-1 antibodyASMA antibodyCFTD antibodyCFTD1 antibodyCFTDM antibodyMPFD antibodyNEM1 antibodyNEM2 antibodyNEM3 antibodynemaline myopathy type 3 antibody
References for Anti-Actin antibody [3F10] (ab18146)
ab18146 has not yet been referenced specifically in any publications.