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Anti-Actin antibody [3F10] (ab18146)

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Overview

  • Product nameAnti-Actin antibody [3F10]See all Actin primary antibodies ...
  • Description
    Mouse monoclonal [3F10] to Actin
  • SpecificityReacts with alpha- smooth muscle as well as alpha-skeletal and alpha-cardiac (sarcomeric) isoform of actin. Reacts with tumors arising from smooth muscle (leiomyosarcomas) as well as skeletal muscle (rhabdomyosarcomas).
  • Tested applicationsIHC-P, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Rat, Horse, Chicken, Dog, Human, Pig
    Predicted to work with: Silk worm
  • Immunogen

    Full length purified Actin (Human).

  • Positive controlMuscle or sarcoma

Properties

Applications

Our Abpromise guarantee covers the use of ab18146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P
ICC/IF
  • Application notesIF: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 1 - 2 µg/ml for 30 min at RT. No special pretreatment is required for immunohistochemical staining of formalin-fixed tissues.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
      Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
      Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
    • Sequence similaritiesBelongs to the actin family.
    • Cellular localizationCytoplasm > cytoskeleton.

      • Target information above from: UniProt accession P68133 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        ACTA antibodyACTA1 antibodyActin alpha skeletal muscle antibody
        Actin antibodyactin, alpha 1, skeletal muscle 1 antibodyactin, alpha 1, skeletal muscle antibodyActin, alpha skeletal muscle antibodyACTS_HUMAN antibodyalpha Actin 1 antibodyalpha skeletal muscle Actin antibodyalpha skeletal muscle antibodyAlpha-actin-1 antibodyASMA antibodyCFTD antibodyCFTD1 antibodyCFTDM antibodyMPFD antibodyNEM1 antibodyNEM2 antibodyNEM3 antibodynemaline myopathy type 3 antibody
      see all

    References for Anti-Actin antibody [3F10] (ab18146)

    ab18146 has not yet been referenced specifically in any publications.

    Publishing research using ab18146 ? Please let us know so that we can cite the reference in this datasheet

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    I would like to use this mouse monoclonal antibody on mouse tissue.

    Thank you for your enquiry. When conducting IHC, it is not advisable to use antibodies which were raised in the same species as the target sample e.g. a mouse mAb against mouse sample. The secondary Ab may recognise endogenous IgG in your sample tissue...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"