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Anti-Actin antibody [AC-40] (Cy3 ®) (ab11004)

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Overview

  • Product nameAnti-Actin antibody [AC-40] (Cy3 ®)See all Actin primary antibodies ...
  • Description
    Mouse monoclonal [AC-40] to Actin (Cy3 ®)
  • ConjugationCy3 ®
  • SpecificityThis antibody does not react with the epitope located on the N-terminus of Actin.
  • Tested applicationsICC/IF, Double Immunodiffusion, IHC-P, IHC-Fr more details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Carp
  • Immunogen

    Synthetic peptide: SGPSIVHRKCF, corresponding to C terminal amino acids 365-375 of Human Actin, attached to a Multiple Antigen Peptide (MAP) backbone.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • EpitopeThis antibody recognizes an epitope located on the C-terminus of actin. This epitope is conserved in all actin isoforms.
  • General notesIf slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.

Properties

Applications

Our Abpromise guarantee covers the use of ab11004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: Use at an assay dependent dilution.
Double Immunodiffusion DID: Use at an assay dependent dilution.
IHC-P IHC-P: Use at an assay dependent dilution.
IHC-Fr IHC-Fr: Use at an assay dependent dilution.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similaritiesBelongs to the actin family.
  • Cellular localizationCytoplasm > cytoskeleton.

    • Target information above from: UniProt accession P68133 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      ACTA antibodyACTA1 antibodyActin alpha skeletal muscle antibody
      Actin antibodyactin, alpha 1, skeletal muscle 1 antibodyactin, alpha 1, skeletal muscle antibodyActin, alpha skeletal muscle antibodyACTS_HUMAN antibodyalpha Actin 1 antibodyalpha skeletal muscle Actin antibodyalpha skeletal muscle antibodyAlpha-actin-1 antibodyASMA antibodyCFTD antibodyCFTD1 antibodyCFTDM antibodyMPFD antibodyNEM1 antibodyNEM2 antibodyNEM3 antibodynemaline myopathy type 3 antibody
    see all

References for Anti-Actin antibody [AC-40] (Cy3 ®) (ab11004)

ab11004 has not yet been referenced specifically in any publications.

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isotype control (Cy3) for this antibody available for sale?

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Unfortunately, the supplying lab let me know that they do not have an Cy3-conjugated isotype control for this antibody.

The following options might work instead:

1) Use an unconjugated isotype co...

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