Anti-Actin antibody [AC-40] (FITC) (ab11005)

Overview

  • Product name
    Anti-Actin antibody [AC-40] (FITC)
    See all Actin primary antibodies
  • Description
    Mouse monoclonal [AC-40] to Actin (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This antibody does not react with the epitope located on the N-terminus of Actin.
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Carp
  • Immunogen

    Synthetic peptide corresponding to Actin aa 365-375 (C terminal). Attached to a Multiple Antigen Peptide (MAP) backbone
    Sequence:

    SGPSIVHRKCF

  • Epitope
    FITC Conjugated Monoclonal anti-Actin recognizes an epitope located on the C-terminal end of actin. This epitope is conserved in all actin isoforms.
  • General notes

    If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Protect from prolonged exposure to light.

     

    F/P Molar Ratio: 3 - 8.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab11005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • Function
    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease
    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similarities
    Belongs to the actin family.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • a actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • Actin alpha skeletal muscle antibody
    • Actin antibody
    • actin, alpha 1, skeletal muscle 1 antibody
    • actin, alpha 1, skeletal muscle antibody
    • Actin, alpha skeletal muscle antibody
    • actina antibody
    • actine antibody
    • ACTS_HUMAN antibody
    • aktin antibody
    • Alpha Actin 1 antibody
    • Alpha skeletal muscle Actin antibody
    • alpha skeletal muscle antibody
    • alpha-actin antibody
    • Alpha-actin-1 antibody
    • ASMA antibody
    • CFTD antibody
    • CFTD1 antibody
    • CFTDM antibody
    • MPFD antibody
    • NEM1 antibody
    • NEM2 antibody
    • NEM3 antibody
    • nemaline myopathy type 3 antibody
    see all

References

This product has been referenced in:
  • Finkenstaedt-Quinn SA  et al. Cytoskeleton dynamics in drug-treated platelets. Anal Bioanal Chem 407:2803-9 (2015). Read more (PubMed: 25701419) »
  • Hauschner H  et al. A unique interaction between alphaIIb and beta3 in the head region is essential for outside-in signaling-related functions of alphaIIbbeta3 integrin. Blood 115:4542-50 (2010). Human . Read more (PubMed: 20308600) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you for contacting us. This antibody is supplied at a concentration of 2.4 mg/ml, so the volume for 50 ug would be ˜21 ul.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice o...

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Thank you for your reply. While we do not generally give discounts for teaching institutions in particular, if you are starting a new lab, moving a lab, or a first-time grant recipient, you may qualify for our New Lab Discount Program:

http:/...

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Thank you for contacting us.

Either of these antibodies would be suitable for your purposes. You could also use our beta Actin antibody ab6277, which has been validated for use on chicken and cow samples in IHC-Fr as well. Beta Actin is commo...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Cell (Fibroblasts)
Specification
Fibroblasts
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Type
Sandwich (Capture)
Username

Abcam user community

Verified customer

Submitted May 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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