Anti-Actin antibody [AC-40] (FITC) (ab11005)
Overview
- Product nameAnti-Actin antibody [AC-40] (FITC)See all Actin primary antibodies ...
- DescriptionMouse monoclonal [AC-40] to Actin (FITC)
- ConjugationFITC
- Conjugation notesF/P Molar Ratio: 3 - 8.
- SpecificityThis antibody does not recognise the epitope on the N-terminal end of actin.
- Tested applicationsICC/IF, IHC-Fr, IHC-P, ELISA more details
- Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Carp
- Immunogen
Synthetic peptide: SGPSIVHRKCF, corresponding to C terminal amino acids 365-375 of Actin, attached to a Multiple Antigen Peptide (MAP) backbone.
- EpitopeFITC Conjugated Monoclonal anti-Actin recognizes an epitope located on the C-terminal end of actin. This epitope is conserved in all actin isoforms.
- General notesIf slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Protect from prolonged exposure to light.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4 -
Concentration information loading... - PurityProtein A purified
- Purification notesMonoclonal anti-Actin is purified using Protein A then conjugated to fluoroscein isothiocyanate isomer I (FITC). It is further purified by gel filtration and contains no detectable free FITC.
- Clonality Monoclonal
- Clone numberAC-40
- IsotypeIgG2a
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab11005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use at an assay dependent dilution. |
| IHC-Fr | IHC-Fr: Use at an assay dependent dilution. |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
| ELISA | ELISA: Use at an assay dependent dilution. |
Target
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions. - Sequence similaritiesBelongs to the actin family.
- Cellular localizationCytoplasm > cytoskeleton.
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Database links
- Entrez Gene: 58 Human
- Entrez Gene: 11459 Mouse
- Entrez Gene: 29437 Rat
- Omim: 102610 Human
- SwissProt: P68133 Human
- SwissProt: P68134 Mouse
- SwissProt: P68136 Rat
- Unigene: 1288 Human
- Unigene: 214950 Mouse
- Unigene: 82732 Rat
see all
Target information above from: UniProt accession
P68133
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ACTA antibodyACTA1 antibodyActin alpha skeletal muscle antibody
- Actin antibodyactin, alpha 1, skeletal muscle 1 antibodyactin, alpha 1, skeletal muscle antibodyActin, alpha skeletal muscle antibodyACTS_HUMAN antibodyalpha Actin 1 antibodyalpha skeletal muscle Actin antibodyalpha skeletal muscle antibodyAlpha-actin-1 antibodyASMA antibodyCFTD antibodyCFTD1 antibodyCFTDM antibodyMPFD antibodyNEM1 antibodyNEM2 antibodyNEM3 antibodynemaline myopathy type 3 antibody
see all
References for Anti-Actin antibody [AC-40] (FITC) (ab11005)
This product has been referenced in:
- Hauschner H et al. A unique interaction between alphaIIb and beta3 in the head region is essential for outside-in signaling-related functions of alphaIIbbeta3 integrin. Blood 115:4542-50 (2010). Human . Read more (PubMed: 20308600) »
- Gajadhar A & Guha A A proximity ligation assay using transiently transfected, epitope-tagged proteins: application for in situ detection of dimerized receptor tyrosine kinases. Biotechniques 48:145-52 (2010). ICC/IF . Read more (PubMed: 20359299) »
