Products:Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
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ab11003 has been referenced in 8 publications.
Publishing research using ab11003? Please let us know so that we can cite the reference in this datasheet
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ab11003 staining human fibroblast cells by ELISA (Sandwich-capture). Cells were blocked in 1% BSA for 1 hour at 25°C. The primary antibody ab11003 (Capture antibody) was diluted 1/3000 and incubated with sample for 12 hours at 4°C. ab5694 was used as secondary and a HRP conjugated goat polyclonal to mouse, diluted 1/1000 was used as detection antibody.
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ab11003 staining Actin in human SW480 cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed and then blocked using 10% serum for 1 hour at 25ºC. Samples were then incubated with primary antibody at 1/50 for 2 hours at 25ºC. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 568 (red) used at a 1/100 dilution.
This image is courtesy of an anonymous Abreview
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