Overview

  • Product nameAnti-Actin antibody [AC-40]See all Actin primary antibodies ...
  • Description
    Mouse monoclonal [AC-40] to Actin
  • SpecificityThe antibody does not react with the epitope on the N-terminal end of actin.
  • Tested applicationsDot Blot, WB, ELISA, IHC-FoFr, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Carp, Snail (Helix pomatia)
  • Immunogen

    Synthetic peptide within Human Actin aa 365-375 (C terminal). The exact sequence is proprietary.
    Sequence:

    SGPSIVHRKCF

  • EpitopeMonoclonal anti-Actin recognizes an epitope located on the C-terminal end of actin. This epitope is conserved in all actin isoforms.
  • General notes

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Applications

Our Abpromise guarantee covers the use of ab11003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot Blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.

1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa.

ELISA Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similaritiesBelongs to the actin family.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACTA antibody
    • ACTA1 antibody
    • Actin alpha skeletal muscle antibody
    • Actin antibody
    • actin, alpha 1, skeletal muscle 1 antibody
    • actin, alpha 1, skeletal muscle antibody
    • Actin, alpha skeletal muscle antibody
    • actina antibody
    • actine antibody
    • ACTS_HUMAN antibody
    • aktin antibody
    • alpha Actin 1 antibody
    • alpha skeletal muscle Actin antibody
    • alpha skeletal muscle antibody
    • alpha-actin antibody
    • Alpha-actin-1 antibody
    • ASMA antibody
    • CFTD antibody
    • CFTD1 antibody
    • CFTDM antibody
    • MPFD antibody
    • NEM1 antibody
    • NEM2 antibody
    • NEM3 antibody
    • nemaline myopathy type 3 antibody
    see all

Anti-Actin antibody [AC-40] images

  • ab11003 staining Actin in Human SW480 cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed and then blocked using 10% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/50 for 2 hours at 25°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 568 (red) used at a 1/100 dilution.

    See Abreview

  • Immunohistochemical analysis of frozen Human tongue tissue, using ab11003.

  • ab11003 staining Human fibroblast cells by ELISA (Sandwich-capture). Cells were blocked in 1% BSA for 1 hour at 25°C. The  primary antibody ab11003 (Capture antibody) was diluted 1/3000 and incubated with sample for 12 hours at 4°C. ab5694 was used as secondary and a HRP conjugated goat polyclonal to mouse, diluted 1/1000 was used as detection antibody.

    See Abreview

References for Anti-Actin antibody [AC-40] (ab11003)

This product has been referenced in:
  • Olsen JR  et al. p63 attenuates epithelial to mesenchymal potential in an experimental prostate cell model. PLoS One 8:e62547 (2013). Read more (PubMed: 23658742) »
  • Yamauchi K  et al. Endoperoxide 4 receptors play a role in evoking the exercise pressor reflex in rats with simulated peripheral artery disease. J Physiol 591:2949-62 (2013). Rat . Read more (PubMed: 23568893) »

See all 13 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse kidney (IMCD))
Loading amount 7.5 µg
Specification Mouse kidney (IMCD)
Gel Running Conditions Reduced Denaturing (4 -12%)
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 24 2012

Thank you for contacting us.

I would not recommend adding SDS or a reducing agent to the sample prior to coating, as this may interfere with the coating efficiency. It is possible that by boiling the protein for 5-10 minutes, the heat denatur...

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The new lot of ab11003 is currently in transit from the production lab to our stock and there is no lot number allocated yet, but I can confirm that this new lot is different from the lot your customer has received originally.

I will send you...

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Thank you for your reply.

Unfortunately we do not have any other lot of ab11003 in stock.

Would your customer be interested in trying an alternative anti-actin from the following list (excluding ab11003 of course) : http://www.abcam...

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Thank you for filling in the questionnaire and for reporting this problem to us. We will use the details you sent about your protocol in order to investigate the source of the problem.

I have looked through your protocol, which looks fine to ...

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Thank you for contacting us.

I am sorry to hear your customeris experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (SW480)
Specification SW480
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 24 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MEF)
Loading amount 8000 cells
Specification MEF
Gel Running Conditions Reduced Denaturing (4-12% Nupage gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 22 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Cell (Fibroblast)
Specification Fibroblast
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Type Sandwich (Capture)
Username

Abcam user community

Verified customer

Submitted Jan 20 2009

Thank you for your enquiry. Ab8226 does not cross-react with ADULT cardiac, smooth, or skeletal muscle actin. Myoblasts are immature muscle cells and looking through the literature it does seem that some people use actin as a loading control. Your cust...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"