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Chicken gizzard Actin
Highly recommended for monitoring total protein load on Western blots.
Our Abpromise guarantee covers the use of ab79667 in the following tested applications.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Use for 2hrs at RT.
|IP||Use at 2 µg/mg of lysate.
Verified on denatured Actin. Use protein G.
|IHC-P||1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use for 30 min with LV’s UltraVision. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
ab79667 has not yet been referenced specifically in any publications.