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Anti-Actin antibody [ACTN05] (Biotin) (ab79667)

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Overview

  • Product nameAnti-Actin antibody [ACTN05] (Biotin)See all Actin primary antibodies ...
  • Description
    Mouse monoclonal [ACTN05] to Actin (Biotin)
  • ConjugationBiotin
  • SpecificityReacts with all six known vertebrate isoforms of actin (MW~42kDa). Also reacts with two cytoplasmic actins (beta, gamma) which are highly homologous to one another but differ from the muscle actins at about 25 amino acid residues.
  • Tested applicationsWB, IP, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Pig, Dictyostelium discoideum, Physarum polycephalum
  • Immunogen

    Chicken gizzard Actin

  • Positive controlHeLa cells. Skeletal muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab79667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
IHC-P
  • Application notesIHC-P: 1/50 for 30 min with LV’s UltraVision. Perform heat mediated antigen retrieval before commencing with IHC staining protocol by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
    IP: Use at 2µg/mg of lysate. Verified on denatured Actin. Use protein G.
    WB: Use at a concentration of 0.5 µg/ml for 2hrs at RT. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
      Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
      Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
    • Sequence similaritiesBelongs to the actin family.
    • Cellular localizationCytoplasm > cytoskeleton.

      • Target information above from: UniProt accession P68133 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        ACTA antibodyACTA1 antibodyActin alpha skeletal muscle antibody
        Actin antibodyactin, alpha 1, skeletal muscle 1 antibodyactin, alpha 1, skeletal muscle antibodyActin, alpha skeletal muscle antibodyACTS_HUMAN antibodyAlpha Actin 1 antibodyAlpha skeletal muscle Actin antibodyalpha skeletal muscle antibodyAlpha-actin-1 antibodyASMA antibodyCFTD antibodyCFTD1 antibodyCFTDM antibodyMPFD antibodyNEM1 antibodyNEM2 antibodyNEM3 antibodynemaline myopathy type 3 antibody
      see all

    References for Anti-Actin antibody [ACTN05] (Biotin) (ab79667)

    ab79667 has not yet been referenced specifically in any publications.

    Publishing research using ab79667 ? Please let us know so that we can cite the reference in this datasheet

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    Interested in uisng ab8227 in sandwich ELISA. Please recomend combinations.

    Thank you for your patience.

    I just received an update form lab regarding the epitope for ab79667 and ab113279. Unfortunately the epitope has not been determined so the information is not available. I am sorry we will not be able to suggest these...

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    Interested in uisng ab8227 in sandwich ELISA. Please recomend combinations.

    Thank you for contacting us.

    I can confirm that I am still waiting for information from lab. I will send you the epitoope details of ab79667 as soon as I receive them.

    The other possible combination of ab8227 in sandwich ELISA would be w...

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    Customer kindly called to inquire about a biotin conjugated primary antibody and associated tissue slide (IHC-P) to use with their streptavidin conjugated reagent.

    Thank you for contacting Abcam regarding finding biotin labeled primary antibodies and control tissues.

    After review of our catalog, my first recommendation is anti-Collagen VI, ab119300. This product is guaranteed in IHC-P and the images on th...

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