Anti-Actin antibody [ACTN05 (C4)] (ab3280)

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19 Specific references

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Publishing research using ab3280? Please let us know so that we can cite the reference in this datasheet

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ab3280 has been referenced in 19 publications.

Tabuchi TM et al. Chromosome-Biased Binding and Gene Regulation by the Caenorhabditis elegans DRM Complex. PLoS Genet 7:e1002074 (2011). WB. PubMed: 21589891
Martrat G et al. Exploring the link between MORF4L1 and risk of breast cancer. Breast Cancer Res 13:R40 (2011). PubMed: 21466675
Mogilenko DA et al. Modified low density lipoprotein stimulates complement C3 expression and secretion via liver X receptor and toll-like receptor 4 activation in human macrophages. J Biol Chem : (2011). WB. PubMed: 22194611
Ha SD et al. Cathepsin B-mediated autophagy flux facilitates the anthrax toxin receptor 2-mediated delivery of anthrax lethal factor into the cytoplasm. J Biol Chem 285:2120-9 (2010). WB; Human, Mouse. PubMed: 19858192
Hamdi MM & Mutungi G Dihydrotestosterone activates the MAPK pathway and modulates maximum isometric force through the EGF receptor in isolated intact mouse skeletal muscle fibres. J Physiol 588:511-25 (2010). WB; Mouse. PubMed: 20008468
Lishko PV et al. Acid extrusion from human spermatozoa is mediated by flagellar voltage-gated proton channel. Cell 140:327-37 (2010). WB; Human. PubMed: 20144758
Williams JM et al. The role of the Wnt family of secreted proteins in rat oval "stem" cell-based liver regeneration: Wnt1 drives differentiation. Am J Pathol 176:2732-42 (2010). WB; Rat. PubMed: 20413689
Overgaard CE et al. Deciliation is associated with dramatic remodeling of epithelial cell junctions and surface domains. Mol Biol Cell 20:102-13 (2009). WB; Dog. PubMed: 19005211
Gitcho MA et al. VCP mutations causing frontotemporal lobar degeneration disrupt localization of TDP-43 and induce cell death. J Biol Chem 284:12384-98 (2009). WB; Human. PubMed: 19237541
Calado RT et al. Sex hormones, acting on the TERT gene, increase telomerase activity in human primary hematopoietic cells. Blood 114:2236-43 (2009). WB; Human. PubMed: 19561322
Jabbour M et al. Discrete domains of MARCH1 mediate its localization, functional interactions, and posttranscriptional control of expression. J Immunol 183:6500-12 (2009). WB; Mouse. PubMed: 19880452
Arnoni CP et al. Regulation of glucose uptake in mesangial cells stimulated by high glucose: role of angiotensin II and insulin. Exp Biol Med (Maywood) 234:1095-101 (2009). WB; Human. PubMed: 19546347
Bolinger C et al. RNA helicase A interacts with divergent lymphotropic retroviruses and promotes translation of human T-cell leukemia virus type 1. Nucleic Acids Res 35:2629-42 (2007). PubMed: 17426138
Reis A et al. Prometaphase APCcdh1 activity prevents non-disjunction in mammalian oocytes. Nat Cell Biol 9:1192-8 (2007). WB; Mouse. PubMed: 17891138
von Einem J et al. The alpha-TIF (VP16) homologue (ETIF) of equine herpesvirus 1 is essential for secondary envelopment and virus egress. J Virol 80:2609-20 (2006). WB; Rabbit. PubMed: 16501071
Philimonenko VV et al. Nuclear actin and myosin I are required for RNA polymerase I transcription. Nat Cell Biol 6:1165-72 (2004). PubMed: 15558034
Luciano L et al. Brush cells of rodent gallbladder and stomach epithelia express neurofilaments. J Histochem Cytochem 51:187-98 (2003). PubMed: 12533527
Hoffner G et al. Perinuclear localization of huntingtin as a consequence of its binding to microtubules through an interaction with beta-tubulin: relevance to Huntington's disease. J Cell Sci 115:941-8 (2002). PubMed: 11870213
Winter H et al. Identification of sucrose synthase as an actin-binding protein. FEBS Lett 430:205-8 (1998). PubMed: 9688539

Publishing research using ab3280? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Western blot - Actin antibody [ACTN05 (C4)] (ab3280)

Predicted band size : 42 kDa

Lanes: 1, 4, 7 - NIH3T3; 2, 5, 8 - MDA MB 321; 3, 6, 9 - HeLa

Lanes: 1-3: 4^oC (1 freeze/thaw); 4-6: 4^oC (2 freeze/thaws); 7-9 : 4^oC (4 freeze/thaws)

Western blot - Actin antibody [ACTN05 (C4)] (ab3280)

All lanes : Anti-Actin antibody [ACTN05 (C4)] (ab3280) at 1/2000 dilution

Lane 1 : Mouse liver whole tissue lysate (mouse 1).
Lane 2 : Mouse liver whole tissue lysate (mouse 2).
Lane 3 : Mouse liver whole tissue lysate (mouse 3).

Lysates/proteins at 50 µg per lane.

Secondary
An HRP-conjugated goat anti-mouse IgG polyclonal. at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 42 kDa

Blocking Step: 5% Milk for 30 minutes at 25°C.

This image is courtesy of an anonymous Abreview

Immunohistochemistry (Frozen sections) - Actin antibody [ACTN05 (C4)] (ab3280)

ab3280 staining Actin in mouse heart tissue sections by IHC-Fr (formaldehyde-fixed frozen sections). Tissue samples were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 25ºC. The sample was incubated with primary antibody (1/250 in PBS-Tween) at 4ºC for 12 hours. An Alexa Fluor^®488-conjugated Goat polyclonal to mouse IgG (1/250) was used as secondary antibody. Nuclei were stained with DAPI.

This image is courtesy of an anonymous Abreview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Actin antibody [ACTN05 (C4)] (ab3280)

ab3280 (1µg/ml) staining actin in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining throughout the tissue.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Immunocytochemistry/ Immunofluorescence - Actin antibody [ACTN05 (C4)] (ab3280)

ICC/IF image of ab3280 stained Hepp cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3280, 10µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunocytochemistry/ Immunofluorescence - Actin antibody [ACTN05 (C4)] (ab3280)

ICC/IF image of ab3280 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3280, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.