Anti-Actin antibody [ACTN05 (C4)] (ab3280)

Dear technical support team:
This customer raises an enquiry about our products; this customer wants to conduct the wb with human serum and mouse serum, therefore, she wants to know is there any suitable antibody can be a loading control in this experiment? Could you please offer any selection to her?
Thanks for your kindly help
Best regards

ANSWER:

Thank you for contacting us.

Anti actin or anti GAPDH antibodies are most suitable to be used as loading control.

Please recommend customer ab3280 or ab9485.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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We need antibodies for EM and FLow cytometry, Human samples:
We would like to see EM results where possible.
Actin
Tubulin
Fibrillin
Mitochondrial marker
Myeloperoxidase

Withsuggested fluoresent secondary antibodies

ANSWER:

Thank you for your recent telephone enquiries.

I have listed below some primary antibodies that may be of interest to you. I can recommend to review the online datasheets for further information.

Actin
I am sorry there are no actin antibodies tested in all of flow cytometry, EM and human samples.

I can provide our testing discount program forab3280if you would like to try this in flow cytometry, please let me know if you would like to receive further information on this. It has already been tested in human samples.

Tubulin

The following tubulin antibody istested in flow cytometry., EM and human samples:

ab44928 Tubulin antibody [DM1A +DM1B]
Mouse monoclonal
Tested in: EM, Flow Cyt, ICC/IF, IHC-P, IP, WB
Reacts with: Fruit fly (Drosophila melanogaster), Human, Mouse, Rat
http://www.abcam.com/index.html?datasheet=44928 (or use the following: http://www.abcam.com/index.html?datasheet=44928).

Fibrillin
The following Filbrillin 1 antibody is tested and guaranteedinflow cytometry, EM and human:
ab3090 Fibrillin 1 antibody [11C1.3]
Mouse monoclonal
Tested in: EM, Flow Cyt, ICC/IF, IF, IHC-Fr, WB
Reacts with: Human
http://www.abcam.com/index.html?datasheet=3090 (or use the following: http://www.abcam.com/index.html?datasheet=3090).

We have the following Fibrillin 2 antibody tested in human. I am sorry this has not yet been tested and guaranteed inEM or flow cytometry. Again, we could offer thetesting discount program for flow cytometry, please let me know if you are interested.
ab53804 Fibrillin 2 antibody
Rabbit polyclonal
Tested in:ELISA
Reacts with: Human
http://www.abcam.com/index.html?datasheet=53804 (or use the following: http://www.abcam.com/index.html?datasheet=53804).

Myeloperoxidase
We have the following, tested in Flow cytometry and human, but they are not tested and guaranteed inEM:
ab25989 Myeloperoxidase antibody [2C7]
Mouse monoclonal
Tested in:ELISA, Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB
Reacts with: Human
http://www.abcam.com/index.html?datasheet=25989 (or use the following: http://www.abcam.com/index.html?datasheet=25989).

ab11729 Myeloperoxidase antibody [2C7] (FITC conjugated)
Mouse monoclonal
Tested in: Flow Cyt, IHC-Fr
Reacts with: Human
http://www.abcam.com/index.html?datasheet=11729 (or use the following: http://www.abcam.com/index.html?datasheet=11729).

ab91237 Myeloperoxidase antibody [7,17] (FITC conjugated)
Mouse monoclonal
Tested in: Flow Cyt
Reacts with: Human
http://www.abcam.com/index.html?datasheet=91237 (or use the following: http://www.abcam.com/index.html?datasheet=91237).

ab11730 Myeloperoxidase antibody [2C7] (Phycoerythrin conjugated)
Mouse monoclonal
Tested in:Flow Cyt
Reacts with: Human
http://www.abcam.com/index.html?datasheet=11730 (or use the following: http://www.abcam.com/index.html?datasheet=11730).

There are many mitochondrial markers you could try:
The following is tested in flow cytometry and human. I am sorry thereare none tested and guaranteed in EM:

ab109798 MS101c Anti-Complex I Immunocapture antibody [18G12BC2]
Mouse monoclonal
Tested in: Flow Cyt, ICC, In-Cell ELISA, IP
Reacts with: Human
http://www.abcam.com/index.html?datasheet=109798 (or use the following: http://www.abcam.com/index.html?datasheet=109798).

ab3298 Mitochondria antibody [MTC02] - Mitochondrial Marker
Mouse monoclonal
Tested in: Flow Cyt, ICC, ICC/IF, IHC-Fr, IHC-FrFl, IHC-P, WB
Reacts with: African Green Monkey, Human, Pig
http://www.abcam.com/index.html?datasheet=3298 (or use the following: http://www.abcam.com/index.html?datasheet=3298).

ab1836 Prohibitin antibody [II-14-10] - Mitochondrial Marker
Mouse monoclonal
Tested in: Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB
Reacts with: Human, Mouse, Rat, Pig
http://www.abcam.com/index.html?datasheet=1836 (or use the following: http://www.abcam.com/index.html?datasheet=1836).

I hope this will be helpful to you. If you would like to let me know which of these antibodies you would like to purchase, I would be pleased to provide you with a list of suitable secondary antibodies for flow cytometry.

Please do not hesitate tolet me know if you would like more details regarding the testing discount program to test ab53804 Fibrillin 2 antibody or ab3280 Tubulin antibody in flow cytometry.

I look forward to hearing from you.

ab3280 does not IP actin

ANSWER:

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Dear sir\madam,

thanks for your cooperation and response.

please we also looking for a secondary antibody:

Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)

and Anti-beta Actin antibody [AC-15] (ab6276).

also please let me know if it is compatible with the three previous

primary antibodies.

ANSWER:

Thank you for contacting us.

The secondary antibody ab6785 (goat anti-mouse IgG, FITC-conjugated) is a suitable secondary for the three primary antibodies you asked about (ab78113, ab90543, and ab3280), as well as ab6276.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

I went to the link you attached but it just takes me to the Abcam Home page---no questionnaire. There was a login page that I filled out that preceded the Home page. The site wanted to set several cookies on my computer, which I refused since I never allow cookies. That probably caused the questionnaire to not pop up.

ANSWER:

You are most likely correct. I have included the questions in this email below. You can reply to this email and fill in the information. I would be happy to give you some assistance. You did reply whether or not you would like to receive a free of charge replacement of ab3280. Please let me know your decision. I look forward to receiving your reply.

BATCH NUMBER ORDER NUMBER

DESCRIPTION OF THE PROBLEM (high background, wrong band size, more bands, no band etc.)

SAMPLE (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)

PRIMARY ANTIBODY (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)

DETECTION METHOD (ECL, ECLPlus etc.)

POSITIVE AND NEGATIVE CONTROLS USED (please specify)

ANTIBODY STORAGE CONDITIONS (temperature/reconstitution etc)

SAMPLE PREPARATION (Buffer/Protease inhibitors/Heating sample etc.)

AMOUNT OF PROTEIN LOADED

ELECTROPHORESIS/GEL CONDITIONS (Reducing or Non-reducing gel, % of the gel etc.)

TRANSFER AND BLOCKING CONDITIONS (Buffer/time period, Blocking agent etc.)

SECONDARY ANTIBODY (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)

HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? HAVE YOU RUN A "NO PRIMARY" CONTROL? DO YOU OBTAIN THE SAME RESULTS EVERY TIME?

WHAT STEPS HAVE YOU ALTERED?