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Western blot - Actin antibody [ACTN05 (C4)] (ab3280)
![Western blot - Actin antibody [ACTN05 (C4)] (ab3280)](/ps/datasheet/images/3/ab3280/Actin-Primary-antibodies-ab3280-1.jpg)
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Product Name
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Actin antibody [ACTN05 (C4)]
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See all Actin antibodies (21)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [ACTN05 (C4)] to Actin
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Immunogen
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Chicken gizzard actin.
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Reacts with
(species key)
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Hu, Ms, Rat, Chk, Cow, D dis, Dog, Phy p, Pig, Rb
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Specificity
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This antibody reacts with all six known vertebrate isoforms of actin. It also reacts with two cytoplasmic actins (beta, lambda) which are highly homologous to one another but which differ from the muscle actins at about 25 amino acidresidues.
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Tested applications
(see key)
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ICC/IF, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution (see Bryce et al). Fix cells with 4% paraformaldehyde and permeabilize with chilled methanol.
IHC-P: Use at a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min).
IP: Use at 2µg/mg of lysate (use Protein G). This has been tested in IP on "denatured" cell lysate (treated with 5% SDS).
WB: Use at a concentration of 0.5 µg/ml for 2hrs at RT. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cells.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> RIP
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Crosslinking
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Tropomyosin
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Troponin
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
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Relevance
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The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 58 (Human)
60 (Human)
71 (Human)
70 (Human)
72 (Human)
| Omim | | 102610 (Human)
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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ACTN05 (C4)
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Produced from ascites then purified by protein G.
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Actin antibody [ACTN05 (C4)] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Actin antibody [ACTN05 (C4)] images, references, products related to ab3280 and other tools.
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Actin antibody [ACTN05 (C4)] images:
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 Western blot - Actin antibody [ACTN05 (C4)] (ab3280)
Predicted band size : 42 kDa
Lanes: 1, 4, 7 - NIH3T3; 2, 5, 8 - MDA MB 321; 3, 6, 9 - HeLa Lanes: 1-3: 4oC (1 freeze/thaw); 4-6: 4oC (2 freeze/thaws); 7-9 : 4oC (4 freeze/thaws)
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References for Actin antibody [ACTN05 (C4)] (ab3280)
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This product has been used in: (two most recent references)
Ha SD et al. Cathepsin B-mediated autophagy flux facilitates the anthrax toxin receptor 2-mediated delivery of anthrax lethal factor into the cytoplasm. J Biol Chem 285:2120-9 (2010). WB; Human, Mouse. PubMed: 19858192
Hamdi MM & Mutungi G Dihydrotestosterone activates the MAPK pathway and modulates maximum isometric force through the EGF receptor in isolated intact mouse skeletal muscle fibres. J Physiol 588:511-25 (2010). WB; Mouse. PubMed: 20008468
See all 19 publication references for this product.
If you publish research using ab3280 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Actin
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Actin antibody [ACTN05 (C4)] - more information
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - Actin antibody [ACTN05 (C4)] (ab3280)](/index.html?datasheet=3280#Actin-Primary-antibodies-ab3280-1.jpg)
