Anti-Actin antibody - C-terminal (ab81144)
- Product nameAnti-Actin antibody - C-terminalSee all Actin primary antibodies ...
- DescriptionSheep polyclonal to Actin - C-terminal
- Tested applicationsWB, IHC-P, IHC-Fr more details
- Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Xenopus laevis, Zebrafish
A synthetic peptide from the C terminal of Human beta, gamma, kappa Actin (ACTB, ACTG, ACTK) conjugated to an immunogenic carrier protein
- Storage instructionsStore at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 1X PBS
- Concentration information loading...
- PurityIgG fraction
- Clonality Polyclonal
Our Abpromise guarantee covers the use of ab81144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use a concentration of 10 - 50 µg/ml. Predicted molecular weight: 42 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.|
|IHC-P||IHC-P: Use a concentration of 10 - 50 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||IHC-Fr: Use a concentration of 10 - 50 µg/ml.|
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
- Sequence similaritiesBelongs to the actin family.
- Cellular localizationCytoplasm > cytoskeleton.
- ACTA antibody
- ACTA1 antibody
- Actin alpha skeletal muscle antibody
- Actin antibody
- actin, alpha 1, skeletal muscle 1 antibody
- actin, alpha 1, skeletal muscle antibody
- Actin, alpha skeletal muscle antibody
- actina antibody
- actine antibody
- ACTS_HUMAN antibody
- aktin antibody
- Alpha Actin 1 antibody
- Alpha skeletal muscle Actin antibody
- alpha skeletal muscle antibody
- alpha-actin antibody
- Alpha-actin-1 antibody
- ASMA antibody
- CFTD antibody
- CFTD1 antibody
- CFTDM antibody
- MPFD antibody
- NEM1 antibody
- NEM2 antibody
- NEM3 antibody
- nemaline myopathy type 3 antibody
References for Anti-Actin antibody - C-terminal (ab81144)
ab81144 has not yet been referenced specifically in any publications.