Anti-Actin antibody - Loading Control (ab1801)

Overview

  • Product nameAnti-Actin antibody - Loading Control
    See all Actin primary antibodies
  • Description
    Rabbit polyclonal to Actin - Loading Control
  • SpecificityThis antibody recognises beta and gamma actin in Human samples. It probably also recognises all the other known forms of Human actin. This antibody detects a single clean band in Human, Mouse, Rat, Chicken and Drosophila samples. In Xenopus laevis a secondary band is detected at about 30kDa. We are unsure whether this is cross-reaction with another actin isoform or merely non-specific. In Cow a doublet is detected, which probably represents different forms of actin.
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human, Xenopus laevis, Fruit fly (Drosophila melanogaster), Zebrafish
    Predicted to work with: Rabbit, Saccharomyces cerevisiae, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Actin.

    (Peptide available as ab13771.)

  • Positive control
    • HeLa whole cell lysate or mouse brain lysate.

Applications

Our Abpromise guarantee covers the use of ab1801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human Actin peptide (ab13771). Block in 5% BSA. Blocking in milk significantly reduces the signal.
  • Application notesIs unsuitable for ICC/IF.
  • Target

    • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
      Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
      Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
    • Sequence similaritiesBelongs to the actin family.
    • Cellular localizationCytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACTA antibody
      • ACTA1 antibody
      • Actin alpha skeletal muscle antibody
      • Actin antibody
      • actin, alpha 1, skeletal muscle 1 antibody
      • actin, alpha 1, skeletal muscle antibody
      • Actin, alpha skeletal muscle antibody
      • actina antibody
      • actine antibody
      • ACTS_HUMAN antibody
      • aktin antibody
      • alpha Actin 1 antibody
      • alpha skeletal muscle Actin antibody
      • alpha skeletal muscle antibody
      • alpha-actin antibody
      • Alpha-actin-1 antibody
      • ASMA antibody
      • CFTD antibody
      • CFTD1 antibody
      • CFTDM antibody
      • MPFD antibody
      • NEM1 antibody
      • NEM2 antibody
      • NEM3 antibody
      • nemaline myopathy type 3 antibody
      see all

    Anti-Actin antibody - Loading Control images

    • All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Brain (Rat) Tissue Lysate
      Lane 3 : Brain (Mouse) Tissue Lysate
      Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 42 kDa
      Observed band size : 42 kDa


      Exposure time : 1 minute

      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab1801 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

    • All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1/1000 dilution

      Lane 1 : Whole cell lysates prepared from HUVEC cells
      Lane 2 : Whole cell lysates prepared from HUVEC cells
      Lane 3 : Whole cell lysates prepared from HUVEC cells
      Lane 4 : Whole cell lysates prepared from HUVEC cells

      Lysates/proteins at 15 µg per lane.

      Secondary
      HRP-conjugated goat polyclonal to rabbit Ig at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 42 kDa


      Exposure time : 30 seconds

      This image is courtesy of an anonymous Abreview

      See Abreview


    • developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 42 kDa

      This image is courtesy of an anonymous Abreview

    • Anti-Actin antibody - Loading Control (ab1801) (Sample: ab73630, 0.1 ug)

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080)
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 42 kDa

    • developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 42 kDa

    References for Anti-Actin antibody - Loading Control (ab1801)

    This product has been referenced in:
    • Moon HJ  et al. Effects of secreted factors in culture medium of annulus fibrosus cells on microvascular endothelial cells: elucidating the possible pathomechanisms of matrix degradation and nerve in-growth in disc degeneration. Osteoarthritis Cartilage 22:344-54 (2014). WB ; Human . Read more (PubMed: 24361793) »
    • Bartoszewski R  et al. Mangiferin has an additive effect on the apoptotic properties of hesperidin in Cyclopia sp. tea extracts. PLoS One 9:e92128 (2014). WB ; Human . Read more (PubMed: 24633329) »

    See all 32 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 40 µg
    Gel Running Conditions Reduced Denaturing (4-20% gradient gel)
    Sample Human Cell lysate - whole cell (HeLa cells)
    Specification HeLa cells
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 21 2014

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Sample African Green Monkey Cell (COS7)
    Specification COS7
    Permeabilization Yes - Triton X 100 0.3%
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jun 03 2013

    Thank you for contacting us.

    I am sorry that this antibody did not perform as stated on the datasheet. Since this order was paid for by credit card, the refund will go back onto the card. To avoid confusion, please ensure your accounts depar...

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    Thank you for taking time to contact us. I am sorry to hear this antibody is not providing satisfactory results.

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    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab8227.

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    Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.

    It sounds like you're seeing "inverse banding" which is white bands on a black background. Usually this means tha...

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    Thank you very much for your reply.

    Please see below for the testing code to try ab121733 with zebrafish samples:

    DISCOUNT CODE: ***
    EXPIRATION DATE: December 12, 2012

    I do hope that this antibody will work well with z...

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    Thank you very much for your reply.

    The testing codes can actuallybe used to receive any primary antibody in our catalog, regardless of target. So, if the WDR81 antibody does not work with zebrafish samples, you can use the code to get any ot...

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    Thank you very much for your email.

    The immunogen is only 46% identical to the zebrafish sequence, but there are a couple of areas of high homology, so it's hard to say whether this antibody might react with zebrafish or not. My guess is that...

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    Thank you very much for you questions.

    Here is your testing code to try ab75591 with zebrafish:

    DISCOUNT CODE: ***
    EXPIRATION DATE: December 6, 2012

    You will need to place your order for this antibody through Kimera as...

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