Anti-Actin antibody - Loading Control (ab1801)


  • Product nameAnti-Actin antibody - Loading Control
    See all Actin primary antibodies
  • Description
    Rabbit polyclonal to Actin - Loading Control
  • SpecificityThis antibody recognises beta and gamma actin in Human samples. It probably also recognises all the other known forms of Human actin. This antibody detects a single clean band in Human, Mouse, Rat, Chicken and Drosophila samples. In Xenopus laevis a secondary band is detected at about 30kDa. We are unsure whether this is cross-reaction with another actin isoform or merely non-specific. In Cow a doublet is detected, which probably represents different forms of actin.
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human, Xenopus laevis, Fruit fly (Drosophila melanogaster), Zebrafish
    Predicted to work with: Rabbit, Saccharomyces cerevisiae, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Actin.

    (Peptide available as ab13771.)

  • Positive control
    • HeLa whole cell lysate or mouse brain lysate. IHC-P - Human Colon FFPE tissue section


Our Abpromise guarantee covers the use of ab1801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human Actin peptide (ab13771). Block in 5% BSA. Blocking in milk significantly reduces the signal.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notesIs unsuitable for ICC/IF.
  • Target

    • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
      Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
      Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
    • Sequence similaritiesBelongs to the actin family.
    • Cellular localizationCytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACTA antibody
      • ACTA1 antibody
      • Actin alpha skeletal muscle antibody
      • Actin antibody
      • actin, alpha 1, skeletal muscle 1 antibody
      • actin, alpha 1, skeletal muscle antibody
      • Actin, alpha skeletal muscle antibody
      • actina antibody
      • actine antibody
      • ACTS_HUMAN antibody
      • aktin antibody
      • alpha Actin 1 antibody
      • alpha skeletal muscle Actin antibody
      • alpha skeletal muscle antibody
      • alpha-actin antibody
      • Alpha-actin-1 antibody
      • ASMA antibody
      • CFTD antibody
      • CFTD1 antibody
      • CFTDM antibody
      • MPFD antibody
      • NEM1 antibody
      • NEM2 antibody
      • NEM3 antibody
      • nemaline myopathy type 3 antibody
      see all

    Anti-Actin antibody - Loading Control images

    • IHC image of ab1801 staining Actin in normal human colon formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1801, 5μl/ml concentration, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab1801 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

    • Secondary antibody - goat anti-rabbit HRP (pre-adsorbed)

    References for Anti-Actin antibody - Loading Control (ab1801)

    This product has been referenced in:
    • Moon HJ  et al. Effects of secreted factors in culture medium of annulus fibrosus cells on microvascular endothelial cells: elucidating the possible pathomechanisms of matrix degradation and nerve in-growth in disc degeneration. Osteoarthritis Cartilage 22:344-54 (2014). WB ; Human . Read more (PubMed: 24361793) »
    • Bartoszewski R  et al. Mangiferin has an additive effect on the apoptotic properties of hesperidin in Cyclopia sp. tea extracts. PLoS One 9:e92128 (2014). WB ; Human . Read more (PubMed: 24633329) »

    See all 37 Publications for this product

    Product Wall

    Application Western blot
    Sample Mouse Tissue lysate - whole (RIPA lysate of whole lung tissue)
    Gel Running Conditions Reduced Denaturing (4-15% gradient)
    Loading amount 100 µg
    Specification RIPA lysate of whole lung tissue
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Ben Thomson

    Verified customer

    Submitted Aug 14 2015

    Application Western blot
    Loading amount 40 µg
    Gel Running Conditions Reduced Denaturing (4-20% gradient gel)
    Sample Human Cell lysate - whole cell (HeLa cells)
    Specification HeLa cells
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jul 21 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Sample African Green Monkey Cell (COS7)
    Specification COS7
    Permeabilization Yes - Triton X 100 0.3%
    Fixative Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jun 03 2013

    Thank you for contacting us.

    I am sorry that this antibody did not perform as stated on the datasheet. Since this order was paid for by credit card, the refund will go back onto the card. To avoid confusion, please ensure your accounts depar...

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    Thank you for taking time to contact us. I am sorry to hear this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product q...

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    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab8227.

    To check the status of the order please contact ou...

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    Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.

    It sounds like you're seeing "inverse banding" which is white bands on a black background. Usually this means tha...

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    Thank you very much for your reply.

    Please see below for the testing code to try ab121733 with zebrafish samples:

    EXPIRATION DATE: December 12, 2012

    I do hope that this antibody will work well with z...

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    Thank you very much for your reply.

    The testing codes can actuallybe used to receive any primary antibody in our catalog, regardless of target. So, if the WDR81 antibody does not work with zebrafish samples, you can use the code to get any ot...

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    Thank you very much for your email.

    The immunogen is only 46% identical to the zebrafish sequence, but there are a couple of areas of high homology, so it's hard to say whether this antibody might react with zebrafish or not. My guess is that...

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