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Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Actin.
(Peptide available as ab13771.)
Our Abpromise guarantee covers the use of ab1801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human Actin peptide (ab13771). Block in 5% BSA. Blocking in milk significantly reduces the signal.|
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab1801 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406
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