Anti-Actin antibody - Loading Control (ab1801)
Overview
- Product nameAnti-Actin antibody - Loading ControlSee all Actin primary antibodies ...
- DescriptionRabbit polyclonal to Actin - Loading Control
- SpecificityThis antibody recognises beta and gamma actin in human samples. It probably also recognises all the other known forms of human actin. This antibody detects a single clean band in Human, Mouse, Rat, Chicken and Drosophila samples. In Xenopus laevis a secondary band is detected at about 30kDa. We are unsure whether this is cross-reaction with another actin isoform or merely non-specific. In Cow a doublet is detected, which probably represents different forms of actin.
- Tested applicationsWB more details
- Species reactivityReacts with: Mouse, Rat, Chicken, Cow, Human, Xenopus laevis, Fruit fly (Drosophila melanogaster), Zebrafish
Predicted to work with: Rabbit, Saccharomyces cerevisiae - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Actin.
(Peptide available as ab13771.)
- Positive controlHeLa whole cell lysate or mouse brain lysate.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab1801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Actin peptide (ab13771). Block in 5% BSA. Blocking in milk significantly reduces the signal. |
Target
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions. - Sequence similaritiesBelongs to the actin family.
- Cellular localizationCytoplasm > cytoskeleton.
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Database links
- Entrez Gene: 58 Human
- Entrez Gene: 11459 Mouse
- Entrez Gene: 29437 Rat
- Omim: 102610 Human
- SwissProt: P68133 Human
- SwissProt: P68134 Mouse
- SwissProt: P68136 Rat
- Unigene: 1288 Human
- Unigene: 214950 Mouse
- Unigene: 82732 Rat
see all
Target information above from: UniProt accession
P68133
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- ACTA antibodyACTA1 antibodyActin alpha skeletal muscle antibody
- Actin antibodyactin, alpha 1, skeletal muscle 1 antibodyactin, alpha 1, skeletal muscle antibodyActin, alpha skeletal muscle antibodyACTS_HUMAN antibodyalpha Actin 1 antibodyalpha skeletal muscle Actin antibodyalpha skeletal muscle antibodyAlpha-actin-1 antibodyASMA antibodyCFTD antibodyCFTD1 antibodyCFTDM antibodyMPFD antibodyNEM1 antibodyNEM2 antibodyNEM3 antibodynemaline myopathy type 3 antibody
see all
Anti-Actin antibody - Loading Control images
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Western blot - Actin antibody (ab1801)All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1/500 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : Rat brain
Lane 3 : Mouse brain
Lane 4 : 3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size : 42 kDa
Observed band size : 42 kDa
Exposure time : 1 minute -
Western blot - Actin antibody (ab1801)All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1/1000 dilution
Lane 1 : Whole cell lysates prepared from HUVEC cells
Lane 2 : Whole cell lysates prepared from HUVEC cells
Lane 3 : Whole cell lysates prepared from HUVEC cells
Lane 4 : Whole cell lysates prepared from HUVEC cells
Lysates/proteins at 15 µg per lane.
Secondary
HRP-conjugated goat polyclonal to rabbit Ig at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42 kDa
Exposure time : 30 secondsThis image is courtesy of an anonymous Abreview
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Western blot - Actin antibody - Loading Control (ab1801)Anti-Actin antibody - Loading Control (ab1801) at 1/1000 dilution
Secondary
An HRP conjugated donkey anti rabbit polyclonal at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42 kDa
Observed band size : 42 kDa
Exposure time : 30 secondsThis image is courtesy of an anonymous Abreview
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Western blotAnti-Actin antibody - Loading Control (ab1801) at 1/1000 dilution +Actin protein (ab73630) at 0.1 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 4 minutes
References for Anti-Actin antibody - Loading Control (ab1801)
This product has been referenced in:
- Bocksteins E et al. Kv3 channels contribute to the delayed rectifier current in small cultured mouse dorsal root ganglion neurons. Am J Physiol Cell Physiol 303:C406-15 (2012). WB ; Mouse . Read more (PubMed: 22673617) »
- Spencer VA et al. Depletion of nuclear actin is a key mediator of quiescence in epithelial cells. J Cell Sci 124:123-32 (2011). ICC, IF ; Mouse . Read more (PubMed: 21172822) »
