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Product Name
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Actin antibody
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See all Actin antibodies (21)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Actin
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Immunogen
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Actin isolated from chicken back muscle.
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Reacts with
(species key)
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Hu, Chk
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Specificity
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Recognizes alpha actin. Crossreacts with beta, gamma and delta actin. No crossreactivity is observed with other cytoskeletal proteins.
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Tested applications
(see key)
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ELISA, IHC-Fr, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
IHC-P: 1/50.
IHC-Fr: 1/50.
For immunohistochemistry incubate ab14130 for 30-60 minutes at room temperature or overnight at 4°C. If DAB is used as a chromogen for immunoperoxidase staining, a further dilution of the primary antibody may be required.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Skeletal muscle/Leiomyosarcoma
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> RIP
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Crosslinking
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Tropomyosin
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Troponin
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
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Relevance
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The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 58 (Human)
60 (Human)
71 (Human)
70 (Human)
72 (Human)
| Omim | | 102610 (Human)
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: 40% Glycerol, 0.1% BSA, PBS, pH 7.2 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.300 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Actin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Actin
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