Anti-Actin antibody (ab15263)
- Product nameAnti-Actin antibodySee all Actin primary antibodies ...
- DescriptionRabbit polyclonal to Actin
- SpecificitySkeletal muscle actin. Also cross-reacts with Smooth muscle actin.
- Tested applicationsWB, IHC-P more details
- Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Rabbit, Guinea pig, Cow
Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (N terminal).
- Positive controlSkeletal muscle.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
Our Abpromise guarantee covers the use of ab15263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use at an assay dependent dilution. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
|IHC-P||IHC-P: 1/200. for 10 min at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.|
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
- Sequence similaritiesBelongs to the actin family.
- Cellular localizationCytoplasm > cytoskeleton.
- ACTA antibodyACTA1 antibodyActin alpha skeletal muscle antibody
- Actin antibodyactin, alpha 1, skeletal muscle 1 antibodyactin, alpha 1, skeletal muscle antibodyActin, alpha skeletal muscle antibodyACTS_HUMAN antibodyAlpha 1 actin antibodyAlpha Actin 1 antibodyAlpha skeletal muscle Actin antibodyalpha skeletal muscle antibodyAlpha-actin-1 antibodyASMA antibodyCFTD antibodyCFTD1 antibodyCFTDM antibodyMPFD antibodyNEM1 antibodyNEM2 antibodyNEM3 antibodynemaline myopathy type 3 antibody
Anti-Actin antibody images
ab15263 staining skeletal muscle Actin by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
Anti-Actin antibody (ab15263) at 1/25 dilution + Raji cell lysate
Predicted band size : 42 kDa
Observed band size : 42 kDa
References for Anti-Actin antibody (ab15263)
This product has been referenced in:
- Tropepe V et al. Neurogenic potential of isolated precursor cells from early post-gastrula somitic tissue. Stem Cells Dev 18:1533-42 (2009). IHC-P ; Mouse . Read more (PubMed: 19326969) »