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Overview

  • Product nameAnti-Actin antibodySee all Actin primary antibodies ...
  • Description
    Rabbit polyclonal to Actin
  • SpecificitySkeletal muscle actin. Also cross-reacts with Smooth muscle actin.
  • Tested applicationsWB, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Guinea pig, Cow
  • Immunogen

    Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (N terminal).

  • Positive controlSkeletal muscle.

Applications

Our Abpromise guarantee covers the use of ab15263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use at an assay dependent dilution. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P IHC-P: 1/200. for 10 min at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similaritiesBelongs to the actin family.
  • Cellular localizationCytoplasm > cytoskeleton.

    • Target information above from: UniProt accession P68133 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      ACTA antibodyACTA1 antibodyActin alpha skeletal muscle antibody
      Actin antibodyactin, alpha 1, skeletal muscle 1 antibodyactin, alpha 1, skeletal muscle antibodyActin, alpha skeletal muscle antibodyACTS_HUMAN antibodyAlpha 1 actin antibodyAlpha Actin 1 antibodyAlpha skeletal muscle Actin antibodyalpha skeletal muscle antibodyAlpha-actin-1 antibodyASMA antibodyCFTD antibodyCFTD1 antibodyCFTDM antibodyMPFD antibodyNEM1 antibodyNEM2 antibodyNEM3 antibodynemaline myopathy type 3 antibody
    see all

Anti-Actin antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - skeletal muscle Actin antibody (ab15263)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - skeletal muscle Actin antibody (ab15263)

    ab15263 staining skeletal muscle Actin by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • Western blot - Actin antibody (ab15263)
    Western blot - Actin antibody (ab15263)
    Anti-Actin antibody (ab15263) at 1/25 dilution + Raji cell lysate

    Predicted band size : 42 kDa
    Observed band size : 42 kDa

References for Anti-Actin antibody (ab15263)

This product has been referenced in:
  • Tropepe V  et al. Neurogenic potential of isolated precursor cells from early post-gastrula somitic tissue. Stem Cells Dev 18:1533-42 (2009). IHC-P ; Mouse . Read more (PubMed: 19326969) »

See 1 Publication for this product

Publishing research using ab15263 ? Please let us know so that we can cite the reference in this datasheet

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Actin antibody

 Excellent
Abreviews
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (skeletal muscle)
Specification skeletal muscle
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH6.0
Permeabilization No
Blocking step BSA as blocking agent for 25 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Apr 26 2010

Immunocytochemistry/ Immunofluorescence abreview for Anti-Actin antibody

 Average
Abreviews
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Differentiated skeletal myoblast)
Specification Differentiated skeletal myoblast
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
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Submitted Dec 04 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"