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Western blot - Actin antibody (ab52220)
(enlarge)
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Product Name
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Actin antibody
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See all Actin antibodies (21)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Actin
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Immunogen
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Synthetic peptide derived from human Actin.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab52220 detects endogenous levels of total Actin protein.
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Tested applications
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
WB: 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from HeLa cells
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> RIP
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Crosslinking
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Tropomyosin
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Troponin
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
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Relevance
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The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 58 (Human)
60 (Human)
71 (Human)
70 (Human)
72 (Human)
| Omim | | 102610 (Human)
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg+2 and Ca+2), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Actin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Actin antibody images, references, products related to ab52220 and other tools.
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Actin antibody images:
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 Western blot - Actin antibody (ab52220)
All lanes : Actin antibody (ab52220) at 1/500 dilution
Lane 1 : extracts from HeLa cells,
Lane 2 : extracts from HeLa cells, with immunizing peptide
Predicted band size : 42 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to Actin
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Actin antibody - more information
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Customer reviews (feedback) regarding Actin antibody |
Customer FAQs regarding Actin antibody |
Protocols for Actin antibody |
Price and availability of products related to Actin antibody |
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Actin antibody - related products:
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Actin antibody - other tools:
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Contact Abcam with a Technical Enquiry about Actin antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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