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Overview

  • Product nameAnti-Actin antibodySee all Actin primary antibodies ...
  • Description
    Rabbit polyclonal to Actin
  • Specificityab52220 detects endogenous levels of total Actin protein.
  • Tested applicationsELISA, WB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from human Actin.

  • Positive controlExtracts from HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab52220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: 1/10000.
WB WB: 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similaritiesBelongs to the actin family.
  • Cellular localizationCytoplasm > cytoskeleton.

    • Target information above from: UniProt accession P68133 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      ACTA antibodyACTA1 antibodyActin alpha skeletal muscle antibody
      Actin antibodyactin, alpha 1, skeletal muscle 1 antibodyactin, alpha 1, skeletal muscle antibodyActin, alpha skeletal muscle antibodyACTS_HUMAN antibodyAlpha Actin 1 antibodyAlpha skeletal muscle Actin antibodyalpha skeletal muscle antibodyAlpha-actin-1 antibodyASMA antibodyCFTD antibodyCFTD1 antibodyCFTDM antibodyMPFD antibodyNEM1 antibodyNEM2 antibodyNEM3 antibodynemaline myopathy type 3 antibody
    see all

Anti-Actin antibody images

  • Western blot - Actin antibody (ab52220)
    Western blot - Actin antibody (ab52220)
    All lanes : Anti-Actin antibody (ab52220) at 1/500 dilution

    Lane 1 : extracts from HeLa cells,
    Lane 2 : extracts from HeLa cells, with immunizing peptide


    Predicted band size : 42 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

References for Anti-Actin antibody (ab52220)

ab52220 has not yet been referenced specifically in any publications.

Publishing research using ab52220 ? Please let us know so that we can cite the reference in this datasheet

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