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Overview

  • Product nameAnti-Actin antibodySee all Actin primary antibodies ...
  • Description
    Rabbit polyclonal to Actin
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Monkey
  • Immunogen

    Full length native protein purified from bovine skeletal muscle

  • Positive controlMouse Heart Tissue Extract, Chicken Heart Tissue Extract

Applications

Our Abpromise guarantee covers the use of ab69512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similaritiesBelongs to the actin family.
  • Cellular localizationCytoplasm > cytoskeleton.

    • Target information above from: UniProt accession P68133 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      ACTA antibodyACTA1 antibodyActin alpha skeletal muscle antibody
      Actin antibodyactin, alpha 1, skeletal muscle 1 antibodyactin, alpha 1, skeletal muscle antibodyActin, alpha skeletal muscle antibodyACTS_HUMAN antibodyalpha Actin 1 antibodyalpha skeletal muscle Actin antibodyalpha skeletal muscle antibodyAlpha-actin-1 antibodyASMA antibodyCFTD antibodyCFTD1 antibodyCFTDM antibodyMPFD antibodyNEM1 antibodyNEM2 antibodyNEM3 antibodynemaline myopathy type 3 antibody
    see all

Anti-Actin antibody images

  • Western blot - Actin antibody (ab69512)
    Western blot - Actin antibody (ab69512)
    All lanes : Anti-Actin antibody (ab69512) at 1/1000 dilution

    Lane 1 : MW marker
    Lane 2 : Mouse heart tissue extract
    Lane 3 : Chicken heart tissue extract


    Predicted band size : 42 kDa
    Observed band size : 42 kDa
    Additional bands at : 35 kDa,55 kDa,70 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Actin antibody (ab69512)

ab69512 has not yet been referenced specifically in any publications.

Publishing research using ab69512 ? Please let us know so that we can cite the reference in this datasheet

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