Actin protein (ab73630)
Constituents: 0.5% (w/v) SDS, 10mM Tris HCl, 1mM DTT, 0.2mM Calcium chloride, 0.2mM ATP, pH 8.
Our Abpromise guarantee covers the use of ab73630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use at an assay dependent concentration. Ab73630 can be used as a WB positive control in conjunction with ab1801.|
|SDS-PAGE||SDS-PAGE: Use at an assay dependent dilution.|
- Actin alpha skeletal muscleactin, alpha 1, skeletal muscleactin, alpha 1, skeletal muscle 1Actin, alpha skeletal muscleACTS_HUMANalpha Actin 1alpha skeletal musclealpha skeletal muscle ActinAlpha-actin-1ASMACFTDCFTD1CFTDMMPFDNEM1NEM2NEM3nemaline myopathy type 3
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
Actin protein images
Anti-Actin antibody - Loading Control (ab1801) at 1/1000 dilution +
Actin protein (ab73630) at 0.1 µg
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 4 minutes
References for Actin protein (ab73630)
ab73630 has not yet been referenced specifically in any publications.