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Overview

  • Product nameActin proteinSee all Actin proteins and peptides ...
  • Protein descriptionUltra pure Full length Actin from Rabbit Muscle having a Molecular mass of 43 kDa.
  • Expression hostBaculovirus infected insect cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesPurity:Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
  • FormLyophilised:Reconstitute in sterile 18MOhm/cm water to not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% (w/v) SDS, 10mM Tris HCl, 1mM DTT, 0.2mM Calcium chloride, 0.2mM ATP, pH 8.
  • Concentration information loading...
  • Additional notesFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab73630 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent concentration. Ab73630 can be used as a WB positive control in conjunction with ab1801.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        ACTAACTA1Actin
        Actin alpha skeletal muscleactin, alpha 1, skeletal muscleactin, alpha 1, skeletal muscle 1Actin, alpha skeletal muscleACTS_HUMANalpha Actin 1alpha skeletal musclealpha skeletal muscle ActinAlpha-actin-1ASMACFTDCFTD1CFTDMMPFDNEM1NEM2NEM3nemaline myopathy type 3
      see all
  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similaritiesBelongs to the actin family.
  • Cellular localizationCytoplasm > cytoskeleton.

    • Target information above from: UniProt accession P68133 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Actin protein images

    • Western blot
      Western blot
      Anti-Actin antibody - Loading Control (ab1801) at 1/1000 dilution + Actin protein (ab73630) at 0.1 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 4 minutes

    References for Actin protein (ab73630)

    ab73630 has not yet been referenced specifically in any publications.

    Publishing research using ab73630 ? Please let us know so that we can cite the reference in this datasheet

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    will test protein ab73630 in WB

    Thank you so much for your colleague's phone call. I am sending the discount code for the actin protein ab73630. DISCOUNT CODE: xxx Expiration date: xxx I am very pleased to hear you would like to accept our offer and test ab73630 in WB. This code will gi...

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    Can I use this protein as control for the beta-actin antibody?

    Thank you for your inquiry. This actin protein (ab73630) was purified from rabbit striated muscle and the purification method used results in a highly purified protein. Unfortunately, the protein of the muscle consists in the alpha-skeletal muscle iso...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"