Recombinant E. coli BirA protein (Active) (ab135015)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 65% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant E. coli BirA protein (Active)
See all BirA proteins and peptides -
Biological activity
ab135015 (10 µg/ml) completely biotinylated 10 µM of substrate MBP-Avi at 30°C for 30 minutes in the standard reaction buffer containing 50 mM Bicine pH 8.3, 10 mM ATP, 10 mM MgOAc, and 50 µM d-Biotin.
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Purity
>= 65 % SDS-PAGE.
Affinity purified. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Escherichia coli -
Sequence
MHHHHHHDYKDDDDKLEVLFQGPKDNTVPLKLIALLANGE FHSGEQLG ETLGMSRAAINKHIQTLRDWGVDVFTVPGKGY SLPEPIQLLNAEKILS QLDDGSVAVLPVIDSTNQYLLDRI GELKSGDACVAEYQHAGRGRRGRK WFSPFGANLYLSMFWR LEQGPAAAIGLSLVIGIVMAEVLRKLGADKVR VKWPNDLY LQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGL TPYLSR WEKLDNFINRPVKLIIGDKEIFGISRGIDKQGAL LLEQDGIIKPWMGG EISLRSAEK -
Predicted molecular weight
38 kDa including tags -
Amino acids
2 to 321 -
Tags
His-DDDDK tag N-Terminus -
Additional sequence information
NP_418404 38 kDa including tags Additional notes: has several mutations plus the extra C terminal sequences compared with NP_418404
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Description
Recombinant E. coli BirA protein (Active)
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab135015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
Useful for covalently biotin-labeling of acceptor proteins for detection and purification assays.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.02% Imidazole
Constituents: 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.79% Tris HCl, 5% Glycerol (glycerin, glycerine), 0.29% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Bifunctional protein birA
- Bio R
- BioR
see all -
Relevance
BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins.
Images
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10% SDS-PAGE analysis of ab135015 (4 µg) stained with Coomassie Blue.
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MBP-Avi biotinylation with His-FLAG-BirA using the standard reaction conditions as outlined in Enzyme Activity.
A. Coomassie blue stained gel showing protein markers:
1. Unbiotinylated MBP-Avi
2. His-FLAG-BirA / MBP-Avi
3. MBP-Avi-biotin control
B. The corresponding Western blot probed with Streptavidin-HRP and developed with DAB (3,3’-Diaminobenzidine) substrate.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab135015 has not yet been referenced specifically in any publications.