Recombinant E. coli RNase H protein (ab91360)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant E. coli RNase H protein
See all RNase H proteins and peptides -
Biological activity
Specific Activity: 100,000 units/mg protein. Unit Definition: 1 unit is defined as the amount of the enzyme that hydrolyzes 1 nmol of the RNA in 3H labeled M13 DNA/RNA hybrid to acid-soluble ribonucleotides in 20 min at 37°C. Endo- and exo-DNase activities and RNase activity were not detected with 100 U/ml RNaseH in 50 ul reaction at 37°C.
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Purity
> 95 % SDS-PAGE.
Greater than 95% protein determined by SDS-PAGE (CBB staining) Endo- and exo-DNase activities and RNase activity were not detected with 100 U/ml RNaseH in 50 ul reaction at 37 degrees C. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Escherichia coli
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Specifications
Our Abpromise guarantee covers the use of ab91360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
To avoid contamination of trace amounts of nucleic acids in BSA, use reaction buffer that does not contain BSA and use RNaseH at higher concentrations.
1 unit is defined as the amount of the enzyme that hydrolyzes 1 nmol of the RNA in 3H-labeled M13 DNA/RNA hybrid to acid-soluble ribonucleotides in 20 min at 37°C.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
pH: 7.5
Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 50% Glycerol (glycerin, glycerine)
50 units/ulThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Ribonuclease H
- Ribonuclease HI
- RNase H
see all -
Relevance
RNase H from E. coli is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA in RNA:DNA duplexes to generate products with 3'-hydroxyl and 5'-phosphate ends.1,2,3 RNase H degrades only the RNA component of the DNA-RNA hybrid (RNA that is hydrogen bonded to a complementary DNA strand). Other enzymes in E. coli which degrade RNA in the DNA-RNA hybrid are DNA polymerase I and exonuclease III, but these degrade either the RNA or DNA of the hybrids. Ribonuclease H will not cleave single-stranded or double-stranded DNA or RNA.1,2 -
Cellular localization
Cytoplasmic
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab91360 has not yet been referenced specifically in any publications.