Recombinant HDAC3+NCOR2 complex protein (ab42631)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: Inhibition Assay
Description
-
Product name
Recombinant HDAC3+NCOR2 complex protein -
Biological activity
Activity: 110 U/ug. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 uM Biomol substrate (Catalog number KI177), and 1 ng/ul HDAC3/NcoR2. Incubation condition: 30 min at 37ºC.
-
Purity
> 90 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected insect cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab42631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Inhibition Assay
-
Form
Liquid -
Additional notes
Co-expressed in a Baculovirus infected Sf9 cell expression system. -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.614% Glutathione, 0.79% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.8004% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- CTG26
- HD 3
- HD3
see all -
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily. -
Post-translational
modificationsSumoylated in vitro. -
Cellular localization
Nucleus. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab42631 has been referenced in 1 publication.
- Demmerle J et al. The nuclear envelope protein emerin binds directly to histone deacetylase 3 (HDAC3) and activates HDAC3 activity. J Biol Chem : (2012). PubMed: 22570481