Recombinant human AMPK alpha 2 + AMPK beta 1 +AMPK gamma 3 protein (ab184881)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, proprietary tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human AMPK alpha 2 + AMPK beta 1 +AMPK gamma 3 protein -
Biological activity
The specific activity of ab184881 was determined to be 360 nmol/min/mg.
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Purity
> 90 % SDS-PAGE.
Assessed by densitometry. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Tags
His tag C-Terminus , proprietary tag N-Terminus -
Additional sequence information
Recombinant full-length human AMPK alpha 2, beta 1 and gamma 3 subunits: Swissprot: P54646, Q9Y478 and Q9UGI9. Predicted molecular weights are 92, 65 and 108 kDa. (NM_006252 , NM_006253 , NM_017431).
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab184881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C.
pH: 7
Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 5' AMP activated protein kinase beta 1 subunit
- 5' AMP activated protein kinase subunit beta 1
- 5' AMP activated protein kinase subunit gamma 3
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Relevance
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. -
Cellular localization
Cytoplasmic and Nuclear
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab184881 has not yet been referenced specifically in any publications.