Recombinant human Acetyl Coenzyme A Carboxylase protein (ab196428)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 70% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human Acetyl Coenzyme A Carboxylase protein
See all Acetyl Coenzyme A Carboxylase proteins and peptides -
Biological activity
Specific Activity: ≥42.5 pmol/min/µg.
A 25 μl Acetyl Coenzyme A carboxylase alpha reaction is conducted in a buffer containing 30 mM HEPES (pH 7.4), 2 mM MgCl2, 2 mM potassium citrate, 1 mM DTT, 100 μM ATP, 12 mM NaHCO3 and 20 μM acetyl-CoA at 30°C for 45 min. ATP reduction is detected.
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Purity
>= 70 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MWWSTLMSILRARSFWKWISTQTVRIIRAVRAHFGGIMDEPSPLAQPLEL NQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLG ISSLQDGLALHIRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFG GNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLK ANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHAS ENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGS GLRVDWQENDFSKRILNVPQELYEKGYVKDVDDGLQAAEEVGYPVMIKAS EGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQIL ADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATPAVFEHMEQCAVKLAK MVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQ IAMGIPLYRIKDIRMMYGVSPWGDSPIDFEDSAHVPCPRGHVIAARITSE NPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSW GENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQMNRIDTGWL DRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSVSNFLHSLERGQVLPA HTLLNTVDVELIYEGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGG LLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVMRSPSAGKLI QYIVEDGGHVFAGQCYAEIEVMKMVMTLTAVESGCIHYVKRPGAALDPGC VLAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNV MNGYCLPDPFFSSKVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPN VEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFM NTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFAL REENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNIL TELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGH QFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYEL NSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTH VASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFSDSPPQS PTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQ QNKATLVDHGIRRLTFLVAQKDFRKQVNYEVDRRFHREFPKFFTFRARDK FEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVG TEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNN TNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAEL KINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGD KQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLW ESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWK MTFKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPR IYVSANSGARIGLAEEIRHMFHVAWVDPEDPYKGYRYLYLTPQDYKRVSA LNSVHCEHVEDEGESRYKITDIIGKEEGIGPENLRGSGMIAGESSLAYNE IITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVY TSNNQLGGIQIMHNNGVTHCTVCDDFEGVFTVLHWLSYMPKSVHSSVPLL NSKDPIDRIIEFVPTKTPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEI MQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQ AGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKF GAYIVDGLRECCQPVLVYIPPQAELRGGSWVVIDSSINPRHMEMYADRES RGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIHLAERLGTPELSTAERKE LENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVISDILDWKTSRT FFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYV WDNNKDLAEWLEKQLTEEDGVHSVIEENIKCISRDYVLKQIRSLVQANPE VAMDSIIHMTQHISPTQRAEVIRILSTMDSPSTDYKDDDDKHHHHHH -
Predicted molecular weight
270 kDa including tags -
Amino acids
1 to 2383 -
Tags
His-DDDDK tag C-Terminus -
Additional sequence information
NM_198834.
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Associated products
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Related Products
- Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab21536)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Acetyl Coenzyme A Carboxylase antibody (ab72046)
- Anti-6X His tag® antibody (ab9108)
Specifications
Our Abpromise guarantee covers the use of ab196428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.79% Tris HCl, 2.9% Sodium chloride, 10% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Contains 100 µg/ml DDDDK peptide.This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- ACAC
- ACACA
- ACACA_HUMAN
see all -
Function
Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. -
Tissue specificity
Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver. -
Pathway
Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1. -
Involvement in disease
Acetyl-CoA carboxylase 1 deficiency -
Sequence similarities
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain. -
Post-translational
modificationsPhosphorylation on Ser-1263 is required for interaction with BRCA1. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab196428 has not yet been referenced specifically in any publications.