Recombinant human BAFF protein (ab198426)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, SDS-PAGE
Description
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Product name
Recombinant human BAFF protein
See all BAFF proteins and peptides -
Biological activity
Measured by its ability to stimulate human B lymphocyte cells. The ED50 of this effect is between 5 - 10 ng/ml
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Purity
>= 98 % SDS-PAGE.
by SDS-PAGE and HPLC analysis -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
AVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKEN KILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCI QNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALK LL -
Predicted molecular weight
17 kDa -
Amino acids
134 to 285 -
Additional sequence information
Full length soluble BAFF
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab198426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
HPLC
SDS-PAGE
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Form
Lyophilized -
Additional notes
Useful for cell culture and for the study of signaling pathways.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 50% PBS, 0.01% DTT
0.2 µm filtered solutionThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
General Info
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Alternative names
- ApoL related ligand TALL 1
- B cell Activating Factor
- B lymphocyte stimulator
see all -
Function
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. -
Tissue specificity
Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
N-glycosylated. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab198426 has not yet been referenced specifically in any publications.