Recombinant human CD19 protein (Fc Chimera Active) (ab167709)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: Flow Cyt, SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human CD19 protein (Fc Chimera Active)
See all CD19 proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA against immobilized FMC63mAb (0.2μg/well). The linear range is 0.15-1.25 μg/mL.
Measured by its binding ability in a functional ELISA against immobilized CD19-Fc at 2 µg/ml ( 100 µl/well ). The linear ranger of 0.04 - 0.75 µg/ml.
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 0.100 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
PEEPLVVKVE EGDNAVLQCL KGTSDGPTQQ LTWSRESPLK PFLKLSLGLP GLGIHMRPLA IWLFIFNVSQ QMGGFYLCQP GPPSEKAWQP GWTVNVEGSG ELFRWNVSDL GGLGCGLKNR SSEGPSSPSG KLMSPKLYVW AKDRPEIWEG EPPCLPPRDS LNQSLSQDLT MAPGSTLWLS CGVPPDSVSR GPLSWTHVHP KGPKSLLSLE LKDDRPARDM WVMETGLLLP RATAQDAGKY YCHRGNLTMS FHLEITARPV LWHWLLRTGG WK -
Predicted molecular weight
56 kDa including tags -
Amino acids
20 to 291 -
Tags
Fc tag C-Terminus -
Additional sequence information
(AAH06338)
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Specifications
Our Abpromise guarantee covers the use of ab167709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Flow Cytometry
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Additional notes
Endotoxin levels determined by the LAL method.
After reconstitution, under sterile conditions, no activity loss observed after storage at -70°C for 3 months.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.40
Constituents: PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 250 µg/ml.
General Info
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Alternative names
- Antibody deficiency due to defect in CD19
- Antibody deficiency due to defect in CD19, included
- AW495831
see all -
Function
Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. -
Involvement in disease
Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsPhosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. -
Cellular localization
Membrane. - Information by UniProt
Images
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Immobilized FMC63 at 1 μg/mL (100 μL/well) binds ab167709.
Linear range: 0.039-0.625 μg/mL (QC tested).
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ab167709, captured on CM5 chip via Anti-Human lgG Fc antibodies surface, binds FMC63 MAb (mouse lgG2a) with an affinity constant of 3.88 nM, as determined in a SPR assay (Biacore T200) (Routinely tested).
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293 cells were transfected with FCM63-scFv and RFP tag.
2x105 of the cells were first incubated with
A. Human Fc tag control.
B. 10 μg/ml of ab167709.
C. 10 μg/ml of ab167709 and FMC63 (Mouse anti-CD19 antibody).
The FITC anti-human IgG Fc was used to analyse with FACS. RFP was used to evaluate CAR (FMC63-scFv) expression and FITC was used to evaluate the binding activity of recombinant human CD19, Fc Tag, low endotoxin.
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Reduced ab167709 on SDS0PAGE, stained overnight with Coomassie Blue.
Purity of protein > 95%
The protein migrates as 56-90 kDa under reducing condition due to glycosylation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab167709 has not yet been referenced specifically in any publications.