Recombinant human CD80 protein (ab180050)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human CD80 protein
See all CD80 proteins and peptides -
Biological activity
Measured by its ability to induce IL 2 secretion by Jurkat human acute T cell leukemia cells. The ED 50 for this effect is typically 0.01 - 0.1 μg/mL in the presence of PHA
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Purity
> 95 % SDS-PAGE.
ab180050 purity was determined by reduced SDS-PAGE and staining overnight with Coomasie Blue. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIW PEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEV TLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNA INTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTT KQEHFPDN -
Predicted molecular weight
25 kDa including tags -
Amino acids
35 to 242 -
Tags
His tag C-Terminus -
Additional sequence information
Extracellular domain. (NP_005182.1)
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab180050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% Trehalose
The formulation is lot specific. Please contact our Technical Support team for detailsThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionThis information is lot specific. Please contact our Technical Support team for details
General Info
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Alternative names
- Activation B7-1 antigen
- B lymphocyte activation antigen B7
- B7
see all -
Function
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. -
Tissue specificity
Expressed on activated B-cells, macrophages and dendritic cells. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
Images
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Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on CM5 Chip can bind Human B7-1, His Tag with an affinity constant of 5.29 µM as determined in a SPR assay (Biacore T200).
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Human B7-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
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Immobilized Human CTLA-4, Fc Tag at 10 µg/mL (100 µL/well) can bind Human B7-1, His Tag with a linear range of 0.156-1.25 µg/mL.
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SDS-PAGE analysis of ab180050 in reducing conditions. Gel stained overnight with Coomassie Blue. Protein migrates as 45 - 75 kDa due to different glycosylation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab180050 has not yet been referenced specifically in any publications.