Recombinant human Flt3 / CD135 protein (ab42610)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Inhibition Assay
Description
-
Product name
Recombinant human Flt3 / CD135 protein
See all Flt3 / CD135 proteins and peptides -
Biological activity
Activity: 20 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30 C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 4 ug /ml recombinant Flt3.
-
Purity
> 80 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Predicted molecular weight
54 kDa -
Tags
His tag N-Terminus
-
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab42610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Inhibition Assay
-
Form
Liquid -
Additional notes
This protein was expressed in a Baculovirus infected Sf9 cell expression system. Expected molecular weight of 54.2 kDa. -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- CD 135
- CD135
- CD135 antigen
see all -
Function
Receptor for the FL cytokine. Has a tyrosine-protein kinase activity. -
Tissue specificity
Bone marrow cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab42610 has not yet been referenced specifically in any publications.