Recombinant human Flt3 / CD135 protein (ab55709)
Key features and details
- Expression system: Insect cells
- Purity: > 75% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human Flt3 / CD135 protein
See all Flt3 / CD135 proteins and peptides -
Biological activity
Specific Activity: 70 nmol/min/mg
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Purity
> 75 % Densitometry.
Affinity purified. -
Expression system
Insect cells -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
73 kDa including tags -
Amino acids
571 to 993 -
Tags
GST tag N-Terminus
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab55709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- CD 135
- CD135
- CD135 antigen
see all -
Function
Receptor for the FL cytokine. Has a tyrosine-protein kinase activity. -
Tissue specificity
Bone marrow cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
Images
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The specific activity of Flt3 / CD135 (ab55709) was determined to be 68 nmol/min/mg as per activity assay protocol and was equivalent to 60 nmol/min/mg as per radiometric assay
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Kinase activity plot using ab55709. Specific activity: 70 nmol/min/mg.
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SDS-PAGE analysis of ab55709, with molecular weight markers. Approximate molecular weight: 73kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab55709 has not yet been referenced specifically in any publications.