Recombinant human GRK3 protein (ab131701)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, WB
Description
-
Product name
Recombinant human GRK3 protein -
Biological activity
The specific activity of ab131701 was determined to be 6 nmol/min/mg
-
Purity
> 95 % SDS-PAGE.
Assessed by densitometry. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Predicted molecular weight
84 kDa including tags -
Amino acids
1 to 554 -
Tags
GST tag N-Terminus
-
Associated products
-
Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab131701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Functional Studies
SDS-PAGE
Western blot
-
Form
Liquid -
Additional notes
ab204859 (GRKtide peptide) can be utilized as a substrate for assessing kinase activity
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.005% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- ADRBK2
- Adrenergic, beta, receptor kinase 2
- ARBK2_HUMAN
see all -
Function
Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain. - Information by UniProt
Images
-
The specific activity of GRK3 (ab131701) was determined to be 7 nmol/min/mg as per activity assay protocol
-
SDS PAGE analysis of ab131701
-
SDS PAGE analysis of ab131701
-
The specific activity of ab131701 was determined to be 6 nmol/min/mg.
-
The purity of ab131701 was determined to be >95% by densitometry.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab131701 has not yet been referenced specifically in any publications.