Recombinant human CCL4/MIP-1 beta protein (ab9676)
Key features and details
- Expression system: Escherichia coli
- Purity: > 99% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human CCL4/MIP-1 beta protein
See all CCL4/MIP-1 beta proteins and peptides -
Biological activity
The biological activity of this product is determined by its ability to chemoattract human monocytes using a concentration range of 5.0 - 20.0 ng/mL.
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Purity
> 99 % SDS-PAGE.
Sterile filtered. Greater than 99% by SDS-PAGE and HPLC analyses. -
Endotoxin level
< 0.100 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
APMGSDPPTA CCFSYTARKL PHNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN -
Predicted molecular weight
8 kDa -
Amino acids
24 to 92
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Specifications
Our Abpromise guarantee covers the use of ab9676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Additional notes
This product was previously labelled as Macrophage Inflammatory Protein 1 beta, MIP1 beta
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionPlease reconstitute in 100ul sterile water.
General Info
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Alternative names
- MIP 1 beta
- Secreted protein G 26
- ACT 2
see all -
Function
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsN-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab9676 has not yet been referenced specifically in any publications.