Recombinant human PDK1 protein (ab125580)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: = 95% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human PDK1 protein
See all PDK1 proteins and peptides -
Biological activity
The specific activity of ab125580 was determined to be 1.7 nmol/min/mg
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Purity
= 95 % Densitometry.
The purity was determined to be 95% by densitometry. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
70 kDa including tags -
Amino acids
1 to 436
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Associated products
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Related Products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab125580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab125602 (Human Pyruvate Dehydrogenase E1-alpha subunit full length protein) can be utilized as a substrate for assessing Kinase activity
This product was previously labelled as Mitochondrial Pyruvate dehydrogenase kinase 1
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial
- HGNC:8809
- Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1
see all -
Function
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. -
Tissue specificity
Expressed predominantly in the heart. -
Sequence similarities
Belongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
Images
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The specific activity of PDK1 (ab125580) was determined to be 1.4 nmol/min/mg as per activity assay protocol and was equivalent to 1.9 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab125580
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SDS PAGE analysis of ab125580
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SDS Page analysis of ab125580
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The specific activity of ab125580 was determined to be 1.7 nmol/min/mg.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab125580 has not yet been referenced specifically in any publications.