Recombinant human PDK3 protein (ab125606)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human PDK3 protein -
Biological activity
The specific activity of ab125606 was determined to be 12 nmol/mg/min.
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Purity
> 90 % Densitometry.
Purity determined to be >90% by densitometry. Affinity purified. Purity is lot specific. Please contact our technical Support team for details. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
69 kDa including tags -
Amino acids
1 to 406 -
Tags
GST tag N-Terminus
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab125606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab125602 (Human Pyruvate Dehydrogenase E1-alpha subunit full length protein) can be utilized as a substrate for assessing Kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- [Pyruvate dehydrogenase [lipoamide] kinase isozyme 3 mitochondrial
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3
- CMTX6
see all -
Function
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. -
Tissue specificity
Found almost exclusively in heart and skeletal muscle. -
Sequence similarities
Belongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab125606 has not yet been referenced specifically in any publications.