Recombinant human PI3K (p110 delta/p85 alpha) protein (Active) (ab125633)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Tags: His tag N-Terminus, His tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
-
Product name
Recombinant human PI3K (p110 delta/p85 alpha) protein (Active) -
Biological activity
The specific activity of ab125633 was equivalent to 2,514 nmol/min/mg as per activity assay protocol.
-
Purity
> 75 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Amino Acid Sequence 1
-
Species
Human -
Predicted molecular weight
111 kDa -
Amino acids
1 to 1044 -
Tags
His tag N-Terminus -
Additional sequence information
NM_005026.
-
Amino Acid Sequence 2
-
Species
Human -
Predicted molecular weight
86 kDa -
Amino acids
2 to 724 -
Tags
His tag N-Terminus -
Additional sequence information
NM_181253.
-
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab125633 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Western blot
Functional Studies
SDS-PAGE
-
Form
Liquid -
Additional notes
Kinase assay.
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.002% PMSF, 0.82% Sodium phosphate, 0.004% DTT, 25% Glycerol, 1.74% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (8)
ab125633 has been referenced in 8 publications.
- Cai M & Sheng L MFG-E8 Exerts Neuroprotection in Neural Stem Cells Induced by Anesthetic Sevoflurane via Regulating the PI3K/AKT Pathways. Stem Cells Int 2022:5609501 (2022). PubMed: 36277041
- Tan L et al. LncRNA GAS5 as miR-26a-5p Sponge Regulates the PTEN/PI3K/Akt Axis and Affects Extracellular Matrix Synthesis in Degenerative Nucleus Pulposus Cells in vitro. Front Neurol 12:653341 (2021). PubMed: 34413821
- Chen Q et al. LncRNA LINC00641 Sponges miR-497-5p to Ameliorate Neural Injury Induced by Anesthesia via Up-Regulating BDNF. Front Mol Neurosci 13:95 (2020). PubMed: 32714145
- Cattley RT et al. Transforming growth factor ß (TGF-ß) receptor signaling regulates kinase networks and phosphatidylinositol metabolism during T-cell activation. J Biol Chem 295:8236-8251 (2020). PubMed: 32358062
- Xu J et al. Knockdown of serpin peptidase inhibitor clade C member 1 inhibits the growth of nasopharyngeal carcinoma cells. Mol Med Rep 19:3658-3666 (2019). PubMed: 30896875
- Liu Y et al. Alisol B 23-acetate inhibits the viability and induces apoptosis of non-small cell lung cancer cells via PI3K/AKT/mTOR signal pathway. Mol Med Rep 20:1187-1195 (2019). PubMed: 31173235
- Shi X et al. Overexpression of Long Noncoding RNA PTENP1 Inhibits Cell Proliferation and Migration via Suppression of miR-19b in Breast Cancer Cells. Oncol Res 26:869-878 (2018). PubMed: 29212574
- Zhang H et al. Inhibition of the PI3K/Akt signaling pathway reverses sorafenib-derived chemo-resistance in hepatocellular carcinoma. Oncol Lett 15:9377-9384 (2018). PubMed: 29928334