Recombinant Human SIGLEC6 protein (Fc Chimera) (ab182674)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human SIGLEC6 protein (Fc Chimera)
See all SIGLEC6 proteins and peptides -
Purity
> 90 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
LLPLLWAGALAQERRFQLEGPESLTVQEGLCVLVPCRLPTTLPASYYGYG YWFLEGADVPVATNDPDEEVQEETRGRFHLLWDPRRKNCSLSIRDARRRD NAAYFFRLKSKWMKYGYTSSKLSVRVMALTHRPNISIPGTLESGHPSNLT CSVPWVCEQGTPPIFSWMSAAPTSLGPRTTQSSVLTITPRPQDHSTNLTC QVTFPGAGVTMERTIQLNVSYAPQKVAISIFQGNSAAFKILQNTSSLPVL EGQALRLLCDADGNPPAHLSWFQGFPALNATPISNTGVLELPQVGSAEEG DFTCR -
Predicted molecular weight
60 kDa including tags -
Amino acids
16 to 320 -
Tags
Fc tag C-Terminus -
Additional sequence information
Fused with Fc fragment of Human IgG1 at the C-terminus.
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Specifications
Our Abpromise guarantee covers the use of ab182674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Additional notes
DTT-reduced SIGLEC6 protein migrates as 80-90 kDa and non-reduced protein migrates as 160-180 kDa.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.6% Tris, 0.75% Glycine, 5% Trehalose
Lyophilized from 0.22 µm filtered solution. -
ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
General Info
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Alternative names
- CD327
- CD33 antigen-like 1
- CD33L
see all -
Function
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. -
Tissue specificity
Expressed at high levels in placenta (cyto- and syncytiotrophoblastic cells) and at lower levels in spleen, peripheral blood leukocytes (predominantly B-cells) and small intestine. -
Sequence similarities
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab182674 has been referenced in 1 publication.
- Luo X et al. Ageing Increases Cardiac Electrical Remodelling in Rats and Mice via NOX4/ROS/CaMKII-Mediated Calcium Signalling. Oxid Med Cell Longev 2022:8538296 (2022). PubMed: 35387264