Recombinant human Wnt1 protein (Active) (ab84080)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, HPLC
Description
-
Product name
Recombinant human Wnt1 protein (Active) -
Biological activity
The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells.
The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.
-
Purity
> 98 % SDS-PAGE.
>98% HPLC. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
ANSSGRWWGI VNVASSTNLL TDSKSLQLVL EPSLQLLSRK QRRLIRQNPG ILHSVSGGLQ SAVRECKWQF RNRRWNCPTA PGPHLFGKIV NRGCRETAFI FAITSAGVTH SVARSCSEGS IESCTCDYRR RGPGGPDWHW GGCSDNIDFG RLFGREFVDS GEKGRDLRFL MNLHNNEAGR TTVFSEMRQE CKCHGMSGSC TVRTCWMRLP TLRAVGDVLR DRFDGASRVL YGNRGSNRAS RAELLRLEPE DPAHKPPSPH DLVYFEKSPN FCTYSGRLGT AGTAGRACNS SSPALDGCEL LCCGRGHRTR TQRVTERCNC TFHWCCHVSC RNCTHTRVLH ECL -
Predicted molecular weight
38 kDa -
Amino acids
28 to 370 -
Additional sequence information
Full length mature protein, without the signal peptide.
-
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab84080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Functional Studies
SDS-PAGE
HPLC
-
Form
Lyophilized -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
ReconstitutionWe recommend initial reconstitution in water alone for full solubility. ab84080 reconstituted is stable for at least 6 months when stored in working aliquots with a carrier protein such as 0.1% BSA at -20oC. Avoid repeated freeze/thaw cycles.
General Info
-
Alternative names
- BMND16
- INT1
- OI15
see all -
Function
Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (10)
ab84080 has been referenced in 10 publications.
- Chen Q et al. Egr-1 mediates low-dose arecoline induced human oral mucosa fibroblast proliferation via transactivation of Wnt5a expression. BMC Mol Cell Biol 21:80 (2020). PubMed: 33167868
- Wang Y et al. Neuroprotection mediated by the Wnt/Frizzled signaling pathway in early brain injury induced by subarachnoid hemorrhage. Neural Regen Res 14:1013-1024 (2019). PubMed: 30762013
- Xie W et al. Sox2 modulates Lef-1 expression during airway submucosal gland development. Am J Physiol Lung Cell Mol Physiol 306:L645-60 (2014). Mouse . PubMed: 24487391
- Cai WY et al. The Wnt-ß-catenin pathway represses let-7 microRNA expression through transactivation of Lin28 to augment breast cancer stem cell expansion. J Cell Sci 126:2877-89 (2013). PubMed: 23613467
- Straussman R et al. Tumour micro-environment elicits innate resistance to RAF inhibitors through HGF secretion. Nature 487:500-4 (2012). PubMed: 22763439
- Duan J et al. Wnt1/ßcatenin injury response activates the epicardium and cardiac fibroblasts to promote cardiac repair. EMBO J : (2011). PubMed: 22085926
- Wexler EM et al. Endogenous Wnt signaling maintains neural progenitor cell potency. Stem Cells 27:1130-41 (2009). PubMed: 19418460
- Joksimovic M et al. Wnt antagonism of Shh facilitates midbrain floor plate neurogenesis. Nat Neurosci 12:125-31 (2009). PubMed: 19122665
- Yang L et al. Phosphorylation of p68 RNA helicase plays a role in platelet-derived growth factor-induced cell proliferation by up-regulating cyclin D1 and c-Myc expression. J Biol Chem 282:16811-9 (2007). PubMed: 17412694
- Williamson D et al. Role for amplification and expression of glypican-5 in rhabdomyosarcoma. Cancer Res 67:57-65 (2007). PubMed: 17210683