Overview

  • Product nameActivin A Human ELISA KitSee all Activin A kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    20.48 pg/ml - 5000 pg/ml
  • Recovery

    83 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.73 82% - 111%
    Serum 77.89 68% - 83%
    Plasma 76.92 67% - 110%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Activin A Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Activin A in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human Activin A coated on a 96-well plate. Standards and samples are pipetted into the wells and Activin A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Activin A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Activin A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceActivin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules.1 Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types.2 Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.
  • Cellular localizationSecreted
  • Alternative names
    • Activin beta A chain
    • Beta 1
    • erythroid differentiation factor
    • erythroid differentiation protein
    • follicle stimulating hormone releasing protein
    • FSH releasing protein
    • Inhibin
    • inhibin beta A chain
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab113316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Activin A Human ELISA Kit images

  • Representative standard curve using ab113316

  • Representative standard curve using ab113316

Protocols

References for Activin A Human ELISA Kit (ab113316)

ab113316 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.

I can confirm that both kits come with strips (12 x 8) and not a plate.

I can fully recommend both kit for the measurement of humanActivin A by ELISA.

The only majordifference is that ab113316 i...

Read More

Thank you for contacting us.

Unfortunately, the clone numbers of the antibodies are proprietary so this information is not available.

The kit will detect recombinant human Activin A and also the Activin A precursor. Both antibodies...

Read More

Thank you for your enquiry and interest.
I regret to inform you that the clone numbers of the antibodies are proprietary so this information is not available.
However, below is some specificity information that the customer may find useful. T...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"