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Overview

  • Product nameActivin A Mouse ELISA KitSee all Activin A kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum, Other biological fluids, Tissue Extracts
  • Assay typeSandwich
  • Sensitivity
    < 10 pg/ml
  • Range
    62.5 pg/ml - 4000 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam's Activin A Mouse ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse Activin A specific-specific monoclonal antibodies were precoated onto 96-well plates. The mouse specific detection monoclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Activin A amount of sample captured in plate.

     

    No detectable cross-reactivity with any other cytokine.

  • Notes

    Store at 4°C for frequent use, at -20°C for infrequent use.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • RelevanceActivin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules.1 Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types.2 Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.
  • Cellular localizationSecreted
    • Alternative names
        Activin beta A chainBeta 1erythroid differentiation factor
        erythroid differentiation proteinfollicle stimulating hormone releasing proteinFSH releasing proteinInhibininhibin beta A chain
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119569 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Activin A Mouse ELISA Kit images

      • Sandwich ELISA - Activin A Mouse ELISA Kit (ab119569)
        Sandwich ELISA - Activin A Mouse ELISA Kit (ab119569)
        This standard curve was generated for demonstration purpose only. A standard curve must be run with each assay.

      Protocols

      References for Activin A Mouse ELISA Kit (ab119569)

      ab119569 has not yet been referenced specifically in any publications.

      Publishing research using ab119569 ? Please let us know so that we can cite the reference in this datasheet

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      Phone call reporting problems in using the Activin A Mouse ELISA Kit (ab119569)

      Vielen Dank für Ihre Geduld.

      Leider waren wir vom Deutschen Technischen Dienst diese Woche nicht erreichbar, mir wurde aber mitgeteilt, dass Sie allerdings von meiner Kollegin Dr. B. kontaktiert wurden.

      Wir benötigen noch ein p...

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      low results in the mouse serum

      Vielen Dank für Ihren Anruf den anderen Tag.

      Das Labor hat mir bestätigt, dass Sie dieses Kit auf Normalem Maus Serum getestet haben, mit Resultaten zwischen 76-320pg/ml. Der Durchschnittswert war 180pg/ml.

      Entspricht dies ci...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"