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Anti-Activin A Receptor Type IB antibody [EPR4815] (ab109300)

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Overview

Product name

Anti-Activin A Receptor Type IB antibody [EPR4815]
See all Activin A Receptor Type IB products (8) ...

Description

Rabbit monoclonal [EPR4815] to Activin A Receptor Type IB

Tested applications

WB, IHC-P, Flow Cyt, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide, corresponding to residues in the extracellular domain of Human Activin A Receptor Type IB.

Positive control

Human brain tissue lysate, Human fetal kidney tissue lysate, SH-SY5Y cell lysate, U87-MG cell lysate, Human fetal liver tissue lysate; Human kidney tissue.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EPR4815

Isotype

IgG1

Research areas

Stem Cells >> Signaling Pathways >> TGF beta >> Surface Molecules
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins

  • Western blot - Activin A Receptor Type IB antibody [EPR4815] (ab109300)Western blot - Activin A Receptor Type IB antibody [EPR4815] (ab109300) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Activin A Receptor Type IB antibody [EPR4815] (ab109300)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Activin A Receptor Type IB antibody [EPR4815] (ab109300) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab109300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/1000 - 1/10000. De...Read more →

    WB: 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa).

  • ShowHide1 Image

    IHC-P

     IHC-P: 1/500 - 1/1000. P...Read more →

    IHC-P: 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.(Heat to 98°C, allow to cool for 10-20 minutes)

  • ShowHide

    Flow Cyt

     Flow Cyt: 1/100 - 1/500. Read more →

    Flow Cyt: 1/100 - 1/500.

  • ICC/IF

     ICC/IF: 1/100 - 1/250.

    ICC/IF: 1/100 - 1/250.

Application notes

Is unsuitable for or IP.

Target

Function

On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Phosphorylates TDP2.

Tissue specificity

Expressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver.

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 GS domain.
Contains 1 protein kinase domain.

Post-translational
modifications

Autophosphorylated.

Cellular localization

Membrane.

Target information above from: UniProt accessionP36896 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Form

There are 3 isoforms produced by alternative splicing. Isoform 1 also known as SKR2-1; isoform 2 as SKR2-2; isoform 3 as SKR2-3.

Alternative names

  • Activin A receptor type 1B antibody
  • Activin A receptor type II like kinase 4 antibody
  • Activin A type 1B receptor antibody
  • Activin A type IB receptor antibody
  • Activin receptor like kinase 4 antibody
  • Activin receptor type 1B antibody
  • Activin receptor type IB antibody
  • Activin receptor type-1B antibody
  • Activin receptor-like kinase 4 antibody
  • ACTR IB antibody
  • ACTR-IB antibody
  • ACTRIB antibody
  • ACV1B_HUMAN antibody
  • ACVR 1B antibody
  • Acvr1b antibody
  • ACVRLK 4 antibody
  • ACVRLK4 antibody
  • ALK 4 antibody
  • ALK-4 antibody
  • ALK4 antibody
  • Serine(threonine) protein kinase receptor R2 antibody
  • Serine/threonine-protein kinase receptor R2 antibody
  • SKR 2 antibody
  • SKR2 antibody
see all

Database links

see all

Anti-Activin A Receptor Type IB antibody [EPR4815] images:

  Western blot - Activin A Receptor Type IB antibody [EPR4815] (ab109300)

Western blot - Activin A Receptor Type IB antibody [EPR4815] (ab109300)

All lanes : Anti-Activin A Receptor Type IB antibody [EPR4815] (ab109300) at 1/1000 dilution

Lane 1 : Human brain tissue lysate
Lane 2 : Human fetal kidney
Lane 3 : SH-SY5Y cell lysate
Lane 4 : U87-MG cell lysate
Lane 5 : Human fetal liver

Lysates/proteins at 10 µg per lane.


Predicted band size : 57 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Activin A Receptor Type IB antibody [EPR4815] (ab109300)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Activin A Receptor Type IB antibody [EPR4815] (ab109300)

ab109300, at 1/500 dilution, staining Activin A receptor type IB in Human kidney tissue by immunohistochemistry.

References for Anti-Activin A Receptor Type IB antibody [EPR4815] (ab109300)

ab109300 has not yet been referenced specifically in any publications.

Publishing research using ab109300? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"