Anti-Acyl-coenzyme A Thioesterase 4 antibody (ab121116)
Key features and details
- Rabbit polyclonal to Acyl-coenzyme A Thioesterase 4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Acyl-coenzyme A Thioesterase 4 antibody -
Description
Rabbit polyclonal to Acyl-coenzyme A Thioesterase 4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence, corresponding to C terminal amino acids 273-416 of Human Acyl-coenzyme A Thioesterase 4.
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Positive control
- Human cerebral cortex tissue; RT 4, EFO 21 and Human Liver lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (By similarity). Succinyl-CoA thioesterase that also hydrolyzes long chain saturated and unsaturated monocarboxylic acyl-CoAs. -
Tissue specificity
Strongest expression in liver and kidney and weaker expression in placenta, heart, and muscle. -
Sequence similarities
Belongs to the C/M/P thioester hydrolase family. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 122970 Human
- Omim: 614314 Human
- SwissProt: Q8N9L9 Human
- Unigene: 49433 Human
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Alternative names
- ACOT4 antibody
- ACOT4_HUMAN antibody
- Acyl-CoA thioesterase 4 antibody
see all
Images
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ab121116 at 1/40 dilution staining Acyl-coenzyme A Thioesterase 4 in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
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All lanes : Anti-Acyl-coenzyme A Thioesterase 4 antibody (ab121116) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : EFO 21 cell lysate
Lane 3 : U 138 MG cell lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121116 has not yet been referenced specifically in any publications.