Anti-Acyl-coenzyme A Thioesterase 8 antibody (ab70104)

Overview

• Product nameAnti-Acyl-coenzyme A Thioesterase 8 antibodySee all Acyl-coenzyme A Thioesterase 8 primary antibodies ...
• Description
  Mouse polyclonal to Acyl-coenzyme A Thioesterase 8
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Acyl-coenzyme A Thioesterase 8 (NP_005460.2, 1 a.a. ~ 319 a.a) full-length human protein.

• Positive controlHuman spleen tissue lysate and Acyl-coenzyme A Thioesterase 8 transfected 293T lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 1X PBS, pH 7.2
• Concentration information loading...
• PurityProtein G purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Fatty acid oxidation

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Fatty acids

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Cardiovascular
  • Lipids / Lipoproteins
  • Fatty Acids
  • Metabolism

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Peroxisome

  • Immunology
  • Immune System Diseases
  • Antiviral Signaling
  • HIV-related

Applications

Target

• FunctionAcyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May mediate Nef-induced down-regulation of CD4. Major thioesterase in peroxisomes. Competes with BAAT (Bile acid CoA: amino acid N-acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs (By similarity). May be involved in the metabolic regulation of peroxisome proliferation.
• Tissue specificityDetected in a T-cell line (at protein level). Ubiquitous.
• Sequence similaritiesBelongs to the C/M/P thioester hydrolase family.
• Cellular localizationPeroxisome.

Target information above from: UniProt accession O14734 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 10005 Human
  • Entrez Gene: 10005 Human
  • Entrez Gene: 10005 Human
  • GenBank: NM_005469.3 Human
  • Omim: 608123 Human
  • SwissProt: O14734 Human
  • SwissProt: O14734 Human
  • SwissProt: O14734 Human

  • Unigene: 444776 Human

  see all

• Alternative names
  ACTEIII antibodyAcot8 antibodyACOT8_HUMAN antibody

  Acyl CoA thioesterase 8 antibodyAcyl-CoA thioesterase 8 antibodyAcyl-coenzyme A thioesterase 8 antibodyCholoyl coenzyme A thioesterase antibodyCholoyl-coenzyme A thioesterase antibodyhACTE III antibodyhACTE-III antibodyhACTEIII antibodyHIV Nef associated acyl CoA thioesterase antibodyHIV-Nef-associated acyl-CoA thioesterase antibodyHNAACTE antibodyhTE antibodyLong chain fatty acyl CoA hydrolase antibodyPalmitoyl CoA hydrolase antibodyPeroxisomal acyl CoA thioesterase 1 antibodyPeroxisomal acyl coenzyme A thioester hydrolase 1 antibodyPeroxisomal acyl-coenzyme A thioester hydrolase 1 antibodyPeroxisomal long chain acyl CoA thioesterase 1 antibodyPeroxisomal long-chain acyl-CoA thioesterase 1 antibodyPTE 1 antibodyPTE 2 antibodyPTE-1 antibodyPTE-2 antibodyPTE1 antibodyPTE2 antibodyThioesterase II antibody

  see all

Anti-Acyl-coenzyme A Thioesterase 8 antibody images

• 

  Western blot - Acyl-coenzyme A Thioesterase 8 antibody (ab70104)
  Anti-Acyl-coenzyme A Thioesterase 8 antibody (ab70104) at 1/500 dilution + human spleen tissue lysate at 25 µg
  
  Secondary
  Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
  
  Predicted band size : 36 kDa
  Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  
• 

  Western blot - Acyl-coenzyme A Thioesterase 8 antibody (ab70104)
  All lanes : Anti-Acyl-coenzyme A Thioesterase 8 antibody (ab70104) at 1/500 dilution
  
  Lane 1 : Acyl-coenzyme A Thioesterase 8 transfected 293T lysate
  Lane 2 : Non-transfected 293T lysate
  
  Lysates/proteins at 25 µg per lane.
  
  Secondary
  Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
  
  Predicted band size : 36 kDa
  Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.