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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
Overview
Product name
Anti-Acyl-coenzyme A Thioesterase 8 antibody
See all Acyl-coenzyme A Thioesterase 8 products (4) ...
Description
Rabbit polyclonal to Acyl-coenzyme A Thioesterase 8
Tested applications
WB, ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide derived from internal sequence of human Acyl-coenzyme A Thioesterase 8.
Positive control
Extracts from HUVEC cells. A549 cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV-related
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 36 kDa.
ELISA
ELISA: 1/40000ELISA: 1/40000
ICC/IF: 1/500 - 1/1000.
Target
Function
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May mediate Nef-induced down-regulation of CD4. Major thioesterase in peroxisomes. Competes with BAAT (Bile acid CoA: amino acid N-acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs (By similarity). May be involved in the metabolic regulation of peroxisome proliferation.
Tissue specificity
Detected in a T-cell line (at protein level). Ubiquitous.
Sequence similarities
Belongs to the C/M/P thioester hydrolase family.
Cellular localization
Peroxisome.
Target information above from: UniProt accessionO14734
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ACTEIII antibody
- hACTE III antibody
- Acot8 antibody
see all
Database links
- Entrez Gene: 10005 Human
- Entrez Gene: 170789 Mouse
- Entrez Gene: 170588 Rat
- GenBank: NM_005469.2 Human
- Omim: 608123 Human
- SwissProt: O14734 Human
- SwissProt: P58137 Mouse
- SwissProt: Q8VHK0 Rat
see all
Anti-Acyl-coenzyme A Thioesterase 8 antibody images:
Western blot - Acyl-coenzyme A Thioesterase 8 antibody (ab75070)
All lanes : Anti-Acyl-coenzyme A Thioesterase 8 antibody (ab75070) at 1/500 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : HUVEC cell lysate with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 36 kDa
Observed band size : 36 kDa
Immunocytochemistry/ Immunofluorescence - Acyl-coenzyme A Thioesterase 8 antibody (ab75070)
Immunofluorescence analysis of Acyl-coenzyme A Thioesterase 8 in A549 cells using ab75070, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
References for Anti-Acyl-coenzyme A Thioesterase 8 antibody (ab75070)
ab75070 has not yet been referenced specifically in any publications.
Publishing research using ab75070? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"