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Western blot - Acyl-coenzyme A Thioesterase 8 antibody (ab75070)
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Immunocytochemistry/ Immunofluorescence - Acyl-coenzyme A Thioesterase 8 antibody (ab75070)
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Product Name
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Acyl-coenzyme A Thioesterase 8 antibody
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See all Acyl-coenzyme A Thioesterase 8 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Acyl-coenzyme A Thioesterase 8
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Immunogen
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Synthetic peptide derived from internal sequence of human Acyl-coenzyme A Thioesterase 8.
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/40000.
ICC/IF: 1/500 - 1/1000.
WB: 1/500 - 1/1000. Predicted molecular weight: 36 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from HUVEC cells. A549 cells.
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Cellular localization
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Peroxisome.
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
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Relevance
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Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Acyl-coenzyme A Thioesterase 8, also known as ACOT8 may mediate Nef-induced down-regulation of CD4. It is a major thioesterase in peroxisomes and competes with BAAT (Bile acid CoA: amino acid N-acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). It shows a preference for medium-length fatty acyl-CoAs and may be involved in the metabolic regulation of peroxisome proliferation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Acyl-coenzyme A Thioesterase 8 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Acyl-coenzyme A Thioesterase 8 antibody images, references, products related to ab75070 and other tools.
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Acyl-coenzyme A Thioesterase 8 antibody images:
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 Western blot - Acyl-coenzyme A Thioesterase 8 antibody (ab75070)
All lanes : Acyl-coenzyme A Thioesterase 8 antibody (ab75070) at 1/500 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : HUVEC cell lysate with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 36 kDa
Observed band size : 36 kDa
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Immunocytochemistry/ Immunofluorescence - Acyl-coenzyme A Thioesterase 8 antibody (ab75070)
Immunofluorescence analysis of Acyl-coenzyme A Thioesterase 8 in A549 cells using ab75070, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
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Search PubMed (MEDLINE) for references to Acyl-coenzyme A Thioesterase 8
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Acyl-coenzyme A Thioesterase 8 antibody - more information
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