Overview

  • Product nameAnti-Acyltransferase like 1 antibody
    See all Acyltransferase like 1 primary antibodies
  • Description
    Mouse polyclonal to Acyltransferase like 1
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human Acyltransferase like 1 (NP_060309.2, amino acids 1-544)

  • Positive control
    • Human pancreas tissue lysate, A431 cell lysate and Acyltransferase like 1 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab88871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa.

Target

  • FunctionPossesses both acyltransferase and acetyltransferase activities. Activity is calcium-dependent. Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF). Also converts lyso-PAF to 1-alkyl-phosphatidylcholine (PC), a major component of cell membranes and a PAF precursor. Under resting conditions, acyltransferase activity is preferred. Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases.
  • PathwayLipid metabolism; phospholipid metabolism.
  • Sequence similaritiesBelongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
    Contains 2 EF-hand domains.
  • DomainThe HXXXXD motif is essential for acyltransferase activity.
  • Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 acylglycerophosphocholine O acyltransferase antibody
    • 1 alkylglycerophosphocholine O acetyltransferase antibody
    • 1-acylglycerophosphocholine O-acyltransferase antibody
    • 1-alkylglycerophosphocholine O-acetyltransferase antibody
    • A330042H22 antibody
    • Acetyl CoA:lyso PAF acetyltransferase antibody
    • Acetyl-CoA:lyso-PAF acetyltransferase antibody
    • Acetyl-CoA:lyso-platelet-activating factor acetyltransferase antibody
    • acyl CoA:lysophosphatidylcholine acyltransferase 2 antibody
    • Acyl-CoA:lysophosphatidylcholine acyltransferase member 1 antibody
    • Acyltransferase like 1A antibody
    • Acyltransferase-like 1 antibody
    • AYTL1 antibody
    • Aytl1a antibody
    • DKFZp686H22112 antibody
    • FLJ20481 antibody
    • lpafat1 antibody
    • LPC acyltransferase 2 antibody
    • LPCAT-2 antibody
    • LPCAT2 antibody
    • Lyso PAF acetyltransferase antibody
    • Lyso platelet activating factor (PAF) acetyltransferase antibody
    • Lyso-PAF acetyltransferase antibody
    • LysoPAFAT antibody
    • LysoPAFAT/LPCAT2 antibody
    • LysoPC acyltransferase 2 antibody
    • Lysophosphatidylcholine acyltransferase 2 antibody
    • OTTMUSP00000029641 antibody
    • PCAT2_HUMAN antibody
    • RGD1563994 antibody
    see all

Anti-Acyltransferase like 1 antibody images

  • Anti-Acyltransferase like 1 antibody (ab88871) at 1 µg/ml + Human pancreas tissue lysate at 50 µg

    Predicted band size : 60 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Anti-Acyltransferase like 1 antibody (ab88871) at 1 µg/ml + A431 cell lysate at 50 µg

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
  • All lanes : Anti-Acyltransferase like 1 antibody (ab88871) at 1 µg/ml

    Lane 1 : Acyltransferase like 1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 60 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-Acyltransferase like 1 antibody (ab88871)

This product has been referenced in:
  • Robichaud PP  et al. Fatty acid remodeling in cellular glycerophospholipids following the activation of human T cells. J Lipid Res 54:2665-77 (2013). Human . Read more (PubMed: 23894206) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa Cells)
Loading amount 20 µg
Specification HeLa Cells
Treatment SiRNA Luciferase 10nM, 20nM, 24 hours
Gel Running Conditions Non-reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Nov 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"