Recombinant Anti-AdSS 2 antibody [EPR12331-52] (ab174842)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12331-52] to AdSS 2
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-AdSS 2 antibody [EPR12331-52]
See all AdSS 2 primary antibodies -
Description
Rabbit monoclonal [EPR12331-52] to AdSS 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human AdSS 2.
Database link: P30520 -
Positive control
- 293T, HT1376 and HepG2 cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12331-52 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 50 kDa.
|
|
IP |
1/10 - 1/100.
|
Notes |
---|
WB
1/1000 - 1/5000. Predicted molecular weight: 50 kDa. |
IP
1/10 - 1/100. |
Target
-
Function
Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP. -
Pathway
Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. -
Sequence similarities
Belongs to the adenylosuccinate synthetase family. -
Cellular localization
Cytoplasm. Partially associated with particulate fractions. - Information by UniProt
-
Database links
- Entrez Gene: 159 Human
- Omim: 103060 Human
- SwissProt: P30520 Human
- Unigene: 498313 Human
-
Alternative names
- (Ade(-)H, complement of antibody
- acidic isozyme antibody
- ADEH antibody
see all
Images
-
All lanes : Anti-AdSS 2 antibody [EPR12331-52] (ab174842) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HT1376 cell lysate
Lane 3 : HepG2 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDa -
Western blot analysis on immunoprecipitation pellet from 293T cell lysate (lane 1) or 1X PBS (lane 2) (negative control), labeling AdSS 2 immunoprecipitated using ab174842 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab174842 has not yet been referenced specifically in any publications.