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Immunohistochemistry (Paraffin-embedded sections) - Adducin antibody (ab58480)
(enlarge)
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Product Name
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Adducin antibody
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See all Adducin antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Adducin
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Immunogen
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Synthetic non-phosphopeptide derived from human ADD1 around the phosphorylation site of threonine 445 (E-K-TP-R-W).
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab58480 detects endogenous levels of total ADD1 protein.
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Tested applications
(see key)
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ELISA, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
IHC-P: 1/50 - 1/100.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Huamn breast carcinoma tissue
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Cellular localization
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Membrane-cytoskeleton-associated protein
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Research areas
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Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
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Relevance
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Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha and beta adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha and gamma adducins are ubiquitously expressed. In contrast, beta adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca2+/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Adducin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Adducin antibody images, references, products related to ab58480 and other tools.
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Adducin antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - Adducin antibody (ab58480)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab58480 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.
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Search PubMed (MEDLINE) for references to Adducin
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Adducin antibody - more information
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Customer reviews (feedback) regarding Adducin antibody |
Customer FAQs regarding Adducin antibody |
Protocols for Adducin antibody |
Price and availability of products related to Adducin antibody |
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Adducin antibody - related products:
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Adducin antibody - other tools:
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Contact Abcam with a Technical Enquiry about Adducin antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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