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Products:Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
Overview
Product name
Anti-Adducin (phospho S662) antibody - Actin associated protein
See all Adducin products (3) ...
Description
Rabbit polyclonal to Adducin (phospho S662) - Actin associated protein
Specificity
Recognizes the phosphorylated serine 662 of human gamma Adducin, phosphorylated serine 724 of alpha Adducin and phosphorylated serine 713 of beta Adducin.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Dog
Predicted to work with
Chicken, Human, Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide: (C)KKFRTPSpFLKKNK conjugated to KLH, corresponding to amino acids 656-668 of Human gamma Adducin.
(C)KKFRTPS pFLKKNK
Positive control
Canine MDCK cells.
General notes
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Properties
Form
Liquid
Storage instructions
Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.1M Tris-glycine. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: Use at a concentration of 1 µg/ml. This concentration will detect phosphorylated alpha Adducin (approximately 120 kDa) and gamma Adducin (approximately 80kD) in RIPA lysates from canine MDCK cells.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha and beta adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha and gamma adducins are ubiquitously expressed. In contrast, beta adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca2+/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
Cellular localization
Membrane-cytoskeleton-associated protein
Alternative names
- ADD1 antibody
- ADD2 antibody
- ADD3 antibody
see all
Database links
- Entrez Gene: 118 Human
- Entrez Gene: 119 Human
- Entrez Gene: 120 Human
- SwissProt: P35611 Human
- SwissProt: P35612 Human
- SwissProt: Q9UEY8 Human
- Unigene: 501012 Human
References for Anti-Adducin (phospho S662) antibody - Actin associated protein (ab14375)
This product has been referenced in:
- Tura Aet al. Efficacy of Rho-kinase inhibition in promoting cell survival and reducing reactive gliosis in the rodent retina. Invest Ophthalmol Vis Sci 50:452-61 (2009). WB; Mouse.Read more (PubMed: 18757509) »
See 1 publication for this product
Publishing research using ab14375? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"