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Products:Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
Overview
Product name
Anti-Adducin (phospho T445) antibody
See all Adducin products (3) ...
Description
Rabbit polyclonal to Adducin (phospho T445)
Specificity
ab58485 detects endogenous levels of Adducin only when phosphorylated at threonine 445.
Tested applications
IHC-P, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide derived from human ADD1 around the phosphorylation site of threonine 445 (E-K-TP-R-W).
Positive control
Human breast carcinoma tissue
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
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Immunohistochemistry (Paraffin-embedded sections) - Adducin (phospho T445) antibody (ab58485)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58485 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/5000ELISA: 1/5000
Target
Relevance
Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha and beta adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha and gamma adducins are ubiquitously expressed. In contrast, beta adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca2+/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
Cellular localization
Membrane-cytoskeleton-associated protein
Alternative names
- ADD 1 antibody
- ADD 2 antibody
- ADD 3 antibody
see all
Database links
- Entrez Gene: 118 Human
- Entrez Gene: 119 Human
- Entrez Gene: 120 Human
- SwissProt: P35611 Human
- SwissProt: P35612 Human
- SwissProt: Q9UEY8 Human
- Unigene: 501012 Human
Anti-Adducin (phospho T445) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - Adducin (phospho T445) antibody (ab58485)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab58485 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.
References for Anti-Adducin (phospho T445) antibody (ab58485)
ab58485 has not yet been referenced specifically in any publications.
Publishing research using ab58485? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"