MitoSciences (MSA02)

Anti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] (ab110322)

Overview

  • Product nameAnti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7]See all Adenine Nucleotide Translocase 1 primary antibodies ...
  • Description
    Mouse monoclonal [5F51BB5AG7] to Adenine Nucleotide Translocase 1
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Caenorhabditis elegans
  • Immunogen

    Recombinant Human Adenine Nucleotide Translocase 1.

  • Positive control
    • Human, Bovine, Rat and Mouse isolated mitochondria; Cultured Human fibroblasts.
  • General notesThis antibody comes with an Incubation Solution which we recommend as a diluent for Western blot. The lab has found that diluting the antibody in this solution gives optimal results. Dilute the antibody to 1 ug/mL in neat Incubation Solution before use.

    The following is a quick reference procedure guide for use specifically with ab110322.

    1. Solubilize samples in SDS-PAGE sample buffer.

    2. Heat sample to 37°C for 30 minutes.

    3. Separate proteins on Tris-glycine 10-22% gradient gel 150 V ~ 2 hrs.

    4. Soak gel in 50 mL CAPS buffer for 30 minutes.

    5. Transfer to 0.45 µm PVDF membrane in CAPS buffer for 2 hrs at 0.15 A CAPS buffer : 10 mM 3-[cyclohexylamino]-1-propane sulfonic acid, pH 11 w/NaOH, 10% methanol ice cold throughout.

    6. Block membrane for 3 hrs - overnight in 5% milk/PBS 4°C.

    7. Wash blot 3 times for 5 mins PBS/0.05% Tween-20 with agitation. Followed by a final rinse in PBS.

    8. Antibody diluted to concentration specified in supplied incubation solution for 2 hrs.

    9. Wash blot 3 times in PBS/0.05% Tween-20 for 5 mins.

    10. Incubate blot with secondary anti-mouse antibody diluted according to manufacturer’s instructions in 1% milk/PBS for 2 hrs.

    11. Wash blot 3 times in PBS/0.05% Tween-20 for 5 mins. Rinse blot in PBS.

    12. Develop Blot: For alkaline phosphatase we recommend BCIP/NBT. For horseradish peroxidase we recommend ECL+.

Properties

Applications

Our Abpromise guarantee covers the use of ab110322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.
ICC/IF Use a concentration of 10 µg/ml. (heat-induced antigen-retrieval improves signal)

Target

  • FunctionCatalyzes the exchange of ADP and ATP across the mitochondrial inner membrane.
  • Involvement in diseaseDefects in SLC25A4 are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 2 (PEOA2) [MIM:609283]. Progressive external ophthalmoplegia is characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism.
  • Sequence similaritiesBelongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAC1 antibody
    • Adenine nucleotide translocator 1 (skeletal muscle) antibody
    • Adenine nucleotide translocator 1 antibody
    • ADP antibody
    • ADP ATP carrier protein 1 antibody
    • ADP ATP carrier protein heart/skeletal muscle isoform T1 antibody
    • ADP/ATP translocase 1 antibody
    • ADT1_HUMAN antibody
    • ANT 1 antibody
    • ANT antibody
    • ANT1 antibody
    • ATP carrier protein 1 antibody
    • ATP carrier protein antibody
    • heart/skeletal muscle isoform T1 antibody
    • PEO2 antibody
    • PEO3 antibody
    • SLC25A4 antibody
    • Solute carrier family 25 member 4 antibody
    • T1 antibody
    see all

Anti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] images

  • All lanes : Anti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] (ab110322) at 1 µg/ml

    Lane 1 : Human heart mitochondria isolate at 5 µg
    Lane 2 : Bovine heart mitochondria isolate at 1 µg
    Lane 3 : Rat heart mitochondria isolate at 10 µg
    Lane 4 : Mouse heart mitochondria isolate at 10 µg


    Predicted band size : 33 kDa
  • ab110322 at 10µg/ml staining Adenine Nucleotide Translocase 1 in cultured Human fibroblasts (fixed and permeabilized) by Immunocytochemistry, followed by a fluorescent goat-anti-mouse IgG.

References for Anti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] (ab110322)

This product has been referenced in:
  • Porter GA  et al. SIRT3 deficiency exacerbates ischemia-reperfusion injury: implication for aged hearts. Am J Physiol Heart Circ Physiol 306:H1602-9 (2014). Read more (PubMed: 24748594) »
  • Duarte FV  et al. Dibenzofuran-induced mitochondrial dysfunction: Interaction with ANT carrier. Toxicol In Vitro 27:2160-2168 (2013). WB . Read more (PubMed: 24008156) »

See all 26 Publications for this product

Product Wall



I can confirm that the incubation solution does not contain any blocking reagent and should only be used to dilute the primary antibody ab110322. (1 ug/mL in neat solution.)

The following is a quick reference procedure guide for use...

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Thank you for contacting us.

During development of this product many different optimization step where attempted. It was found that the protocol detailed on the datasheet gave the best results with this product. We highly recommend using t...

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Thank you very much for your interest in ab110322 (MSA02) and sorry about the confusion with the catalog numbers.

To our knowledge, the Anti-Adenine Nucleotide Translocase 1 antibodyab110322 (or MSA02)has not been tested in immunoprecipitation...

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Thank you for contacting us and your interest in our products. There are a few differentpositive controlsI can suggest for using withthese antibodies.

I can sumarise as follows:

The anti-eNos(phosphoS847) antibody, ab16650,has been ...

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Thank you for contacting us.

I can confirm that the solution is an antibody diluent. The lab has found that diluting the antibody in this solution gives an optimal signal, and they recommend diluting the antibody to 1 ug/mL in neat incubatio...

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Thank you for your phone call.
I have attached the specific WB protocol for ab110322 to this email and am working to have it added to our datasheet. Our MitoSciences team does not have a specific protocol for ab110413, but I have attached their ge...

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Thank you for your call today, and I apologize that we did not already have this information in our notes.
I've gotten in touch with the lab, and this is intended to be an antibody diluent for Western blot. The lab has found that diluting the anti...

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I am very pleased to hear you would like to accept our offer and test ab110322 and ab109864 in IP on mouse tissue. This code will give you 1 free PRIMARY ANTIBODY for each code before the expiration date. To redeem this offer, please submit an Abreview...

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Thank you for contacting us.

We unfortunately at this time Anti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] (ab110322) has not been tested for use in IP. However, this does not mean that it will not work, just that we have not t...

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Thank you for contacting us and your interest in our products.
We unfortunately have no data to show that Anti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] (ab110322) is suitable for use in IHC-P. However, this does not mean that it will...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"