Anti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] (ab110322)

Overview

• Product nameAnti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7]See all Adenine Nucleotide Translocase 1 primary antibodies ...
• Description
  Mouse monoclonal [5F51BB5AG7] to Adenine Nucleotide Translocase 1
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Cow, Human, Caenorhabditis elegans
  
• Immunogen

  Recombinant Human Adenine Nucleotide Translocase 1.

• Positive controlHuman, Bovine, Rat and Mouse isolated mitochondria; Cultured Human fibroblasts.
• General notesThis antibody comes with an Incubation Solution which we recommend as a diluent for Western blot. The lab has found that diluting the antibody in this solution gives optimal results. Dilute the antibody to 1 ug/mL in neat Incubation Solution before use.

  The following is a quick reference procedure guide for use specifically with ab110322.
  
  1. Solubilize samples in SDS-PAGE sample buffer.
  
  2. Heat sample to 37°C for 30 minutes.
  
  3. Separate proteins on Tris-glycine 10-22% gradient gel 150 V ~ 2 hrs.
  
  4. Soak gel in 50 mL CAPS buffer for 30 minutes.
  
  5. Transfer to 0.45 µm PVDF membrane in CAPS buffer for 2 hrs at 0.15 A CAPS buffer : 10 mM 3-[cyclohexylamino]-1-propane sulfonic acid, pH 11 w/NaOH, 10% methanol ice cold throughout.
  
  6. Block membrane for 3 hrs - overnight in 5% milk/PBS 4°C.
  
  7. Wash blot 3 times for 5 mins PBS/0.05% Tween-20 with agitation. Followed by a final rinse in PBS.
  
  8. Antibody diluted to concentration specified in supplied incubation solution for 2 hrs.
  
  9. Wash blot 3 times in PBS/0.05% Tween-20 for 5 mins.
  
  10. Incubate blot with secondary anti-mouse antibody diluted according to manufacturer’s instructions in 1% milk/PBS for 2 hrs.
  
  11. Wash blot 3 times in PBS/0.05% Tween-20 for 5 mins. Rinse blot in PBS.
  
  12. Develop Blot: For alkaline phosphatase we recommend BCIP/NBT. For horseradish peroxidase we recommend ECL+.

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.02% Sodium azide
  Constituent: HBS
• Concentration information loading...
• PurityIgG fraction
• Purification notesab110322 Was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
• Clonality Monoclonal
• Clone number5F51BB5AG7
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Types of disease
  • Cancer

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Mitochondria

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Associated Proteins

Applications

Target

• FunctionCatalyzes the exchange of ADP and ATP across the mitochondrial inner membrane.
• Involvement in diseaseDefects in SLC25A4 are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 2 (PEOA2) [MIM:609283]. Progressive external ophthalmoplegia is characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism.
• Sequence similaritiesBelongs to the mitochondrial carrier family.
  Contains 3 Solcar repeats.
• Cellular localizationMitochondrion inner membrane.

Target information above from: UniProt accession P12235 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 282478 Cow
  • Entrez Gene: 291 Human
  • Entrez Gene: 11739 Mouse
  • Entrez Gene: 85333 Rat
  • Omim: 103220 Human
  • SwissProt: P02722 Cow
  • SwissProt: P12235 Human
  • SwissProt: P48962 Mouse

  • SwissProt: Q05962 Rat
  • Unigene: 246506 Human
  • Unigene: 16228 Mouse
  • Unigene: 4092 Rat

  see all

• Alternative names
  AAC1 antibodyAdenine nucleotide translocator 1 (skeletal muscle) antibodyAdenine nucleotide translocator 1 antibody

  ADP antibodyADP ATP carrier protein 1 antibodyADP ATP carrier protein heart/skeletal muscle isoform T1 antibodyADP/ATP translocase 1 antibodyADT1_HUMAN antibodyANT 1 antibodyANT antibodyANT1 antibodyATP carrier protein 1 antibodyATP carrier protein antibodyheart/skeletal muscle isoform T1 antibodyPEO2 antibodyPEO3 antibodySLC25A4 antibodySolute carrier family 25 member 4 antibodyT1 antibody

  see all

Anti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] images

• 

  Western blot - Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] (ab110322)
  All lanes : Anti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] (ab110322) at 1 µg/ml
  
  Lane 1 : Human heart mitochondria isolate at 5 µg
  Lane 2 : Bovine heart mitochondria isolate at 1 µg
  Lane 3 : Rat heart mitochondria isolate at 10 µg
  Lane 4 : Mouse heart mitochondria isolate at 10 µg
  
  
  Predicted band size : 33 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] (ab110322)
  ab110322 at 10µg/ml staining Adenine Nucleotide Translocase 1 in cultured Human fibroblasts (fixed and permeabilized) by Immunocytochemistry, followed by a fluorescent goat-anti-mouse IgG.