![Western blot - Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] (ab110322) image](#Adenine-Nucleotide-Translocase-1-Primary-antibodies-ab110322-1.jpg)
![Immunocytochemistry/ Immunofluorescence - Anti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] (ab110322) image](#Adenine-Nucleotide-Translocase-1-Primary-antibodies-ab110322-3.gif)
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Product name
Anti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7]
See all Adenine Nucleotide Translocase 1 products (5) ...
Description
Mouse monoclonal [5F51BB5AG7] to Adenine Nucleotide Translocase 1
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human, Caenorhabditis elegans
Immunogen
Recombinant Human Adenine Nucleotide Translocase 1.
Positive control
Human, Bovine, Rat and Mouse isolated mitochondria; Cultured Human fibroblasts.
General notes
This antibody comes with an Incubation Solution which we recommend as a diluent for Western blot. The lab has found that diluting the antibody in this solution gives optimal results. Dilute the antibody to 1 ug/mL in neat Incubation Solution before use.
The following is a quick reference procedure guide for use specifically with ab110322.
1. Solubilize samples in SDS-PAGE sample buffer.
2. Heat sample to 37°C for 30 minutes.
3. Separate proteins on Tris-glycine 10-22% gradient gel 150 V ~ 2 hrs.
4. Soak gel in 50 mL CAPS buffer for 30 minutes.
5. Transfer to 0.45 µm PVDF membrane in CAPS buffer for 2 hrs at 0.15 A CAPS buffer : 10 mM 3-[cyclohexylamino]-1-propane sulfonic acid, pH 11 w/NaOH, 10% methanol ice cold throughout.
6. Block membrane for 3 hrs - overnight in 5% milk/PBS 4°C.
7. Wash blot 3 times for 5 mins PBS/0.05% Tween-20 with agitation. Followed by a final rinse in PBS.
8. Antibody diluted to concentration specified in supplied incubation solution for 2 hrs.
9. Wash blot 3 times in PBS/0.05% Tween-20 for 5 mins.
10. Incubate blot with secondary anti-mouse antibody diluted according to manufacturer’s instructions in 1% milk/PBS for 2 hrs.
11. Wash blot 3 times in PBS/0.05% Tween-20 for 5 mins. Rinse blot in PBS.
12. Develop Blot: For alkaline phosphatase we recommend BCIP/NBT. For horseradish peroxidase we recommend ECL+.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
ab110322 Was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
5F51BB5AG7
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Signal Transduction >> Metabolism >> Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.
ICC/IF: Use a concentration of 10 µg/ml. (heat-induced antigen-retrieval improves signal)
Target
Function
Catalyzes the exchange of ADP and ATP across the mitochondrial inner membrane.
Involvement in disease
Defects in SLC25A4 are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 2 (PEOA2) [MIM:609283]. Progressive external ophthalmoplegia is characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism.
Sequence similarities
Belongs to the mitochondrial carrier family.
Contains 3 Solcar repeats.
Cellular localization
Mitochondrion inner membrane.
Target information above from: UniProt accessionP12235
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- heart/skeletal muscle isoform T1 antibody
- Adenine nucleotide translocator 1 (skeletal muscle) antibody
- Adenine nucleotide translocator 1 antibody
see all
Database links
- Entrez Gene: 282478 Cow
- Entrez Gene: 291 Human
- Entrez Gene: 291 Human
- Entrez Gene: 11739 Mouse
- Entrez Gene: 85333 Rat
- Entrez Gene: 85333 Rat
- Omim: 103220 Human
- SwissProt: P02722 Cow
see all
Anti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] images:
Western blot - Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] (ab110322)
![Western blot - Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] (ab110322)](/ps/datasheet/images/110/ab110322/Adenine-Nucleotide-Translocase-1-Primary-antibodies-ab110322-1.jpg)
All lanes : Anti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] (ab110322) at 1 µg/ml
Lane 1 : Human heart mitochondria isolate at 5 µg
Lane 2 : Bovine heart mitochondria isolate at 1 µg
Lane 3 : Rat heart mitochondria isolate at 10 µg
Lane 4 : Mouse heart mitochondria isolate at 10 µg
Predicted band size : 33 kDa
Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
Immunocytochemistry/ Immunofluorescence - Anti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] (ab110322)
![Immunocytochemistry/ Immunofluorescence - Anti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] (ab110322)](/ps/datasheet/images/110/ab110322/Adenine-Nucleotide-Translocase-1-Primary-antibodies-ab110322-3.gif)
ab110322 at 10µg/ml staining Adenine Nucleotide Translocase 1 in cultured Human fibroblasts (fixed and permeabilized) by Immunocytochemistry, followed by a fluorescent goat-anti-mouse IgG.
References for Anti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] (ab110322)
This product has been referenced in:
- Lynn EG et al. Transient upregulation of PGC-1alpha diminishes cardiac ischemia tolerance via upregulation of ANT1. J Mol Cell Cardiol 49:693-8 (2010).Read more (PubMed: 20600099) »
- Brys K et al. Disruption of insulin signalling preserves bioenergetic competence of mitochondria in ageing Caenorhabditis elegans. BMC Biol 8:91 (2010).Read more (PubMed: 20584279) »
See all 20 publications for this product
Publishing research using ab110322? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"