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MitoSciences (MSA01c)

Anti-Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7] (ab109864)

Submit an Abreview Q&A (3)Specific References (4)

Overview

  • Product nameAnti-Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7]
  • Description
    Mouse monoclonal [5F51BB5AG7] to Adenine Nucleotide Translocase Immunocapture
  • Tested applicationsIP more details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Recombinant Human Adenine Nucleotide Translocase

  • Positive controlBovine and Human heart mitochondria

Properties

Applications

Our Abpromise guarantee covers the use of ab109864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP IP: Use at 100 µg/mg of lysate.

Target

  • RelevanceANT is a reversible transporter of ATP and ADP. During oxidative phosphorylation (OXPHOS) the protein exchanges ATP out for ADP in, however, in the absence of OXPHOS ANT works in reverse to maintain mitochondrial membrane potential. A subset of patients with heart related diseases have been shown to harbor mutations in the ANT gene or to develop an autoimmune reaction against the protein. There are a number of proteins found in mitochondria that, under circumstances favoring apoptosis, coalesce to form the so-called permeability transition pore. The proteins identified as a part of the PTP include cytosolic hexokinase, outer membrane porin (also called the voltage dependent anion channel or VDAC), adenylate cyclase in the intermembrane space, the adenine nucleotide transporter (ANT) and the peripheral benzodiazepine receptor protein of the inner membrane along with cyclophilin D of the matrix space. Hexokinase, porin and ANT all occur as isoforms and it remains to be determined whether there is specificity of these different forms for the PTP.
  • Cellular localizationMitochondrial

Anti-Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7] images

  • Immunoprecipitation - Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7] (ab109864)
    Immunoprecipitation - Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7] (ab109864)
    Immunocapture of Adenine Nucleotide Translocase from Bovine heart mitochondria using ab109864 at a concentration of 100 µg/mg lysate.

References for Anti-Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7] (ab109864)

This product has been referenced in:
  • Mailloux RJ  et al. Hexokinase II acts through UCP3 to suppress mitochondrial reactive oxygen species production and maintain aerobic respiration. Biochem J 437:301-11 (2011). Read more (PubMed: 21554247) »
  • Shulga N  et al. Sirtuin-3 deacetylation of cyclophilin D induces dissociation of hexokinase II from the mitochondria. J Cell Sci 123:894-902 (2010). Read more (PubMed: 20159966) »

See all 4 Publications for this product

Publishing research using ab109864 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

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thanks for the information. I would love to try the IP conditions and would love to test both antibodies ab110322 as well as ab109864 for IP purpose on mouse tissue. Do you know the peptide the Ab is directed against? I would like to test for interaction...

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I am very pleased to hear you would like to accept our offer and test ab110322 and ab109864 in IP on mouse tissue. This code will give you 1 free PRIMARY ANTIBODY for each code before the expiration date. To redeem this offer, please submit an Abreview fo...

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can this antibody also used for IP purpose? (Anti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] (ab110322)). or does the one recommended for IP purpose, recognize mouse? Against which peptide is this one directed at (MSA01c) thanks a lot,

Thank you for contacting us.

We unfortunately at this time Anti-Adenine Nucleotide Translocase 1 antibody [5F51BB5AG7] (ab110322) has not been tested for use in IP. However, this does not mean that it will not work, just that we have not test...

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Is the recommended concentration for IP correct (100 ug per 1 mg of lysate)? I frequently use 1-5 ug of antibody per mg of mitochondrial lysate. Is the antibody suitable for Western blot?

Thanks for your phone call last week and I apologize for the extended delay getting back to you. There is an error on the datasheet, and the recommended antibody ratio of 100 ug per mg of sample is actually for a different Mitosciences product in which th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"