Anti-Adenosine A3 Receptor/A3AR antibody (ab197350)
Key features and details
- Rabbit polyclonal to Adenosine A3 Receptor/A3AR
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Adenosine A3 Receptor/A3AR antibody
See all Adenosine A3 Receptor/A3AR primary antibodies -
Description
Rabbit polyclonal to Adenosine A3 Receptor/A3AR -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment corresponding to Human Adenosine A3 Receptor/A3AR (internal sequence).
Database link: P33765 -
Positive control
- Human esophagus cancer tissue; Mouse testis tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/100.
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WB |
1/500 - 1/1000. Predicted molecular weight: 36 kDa.
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Notes |
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IHC-P
1/20 - 1/100. |
WB
1/500 - 1/1000. Predicted molecular weight: 36 kDa. |
Target
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Function
Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. Possible role in reproduction. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsPhosphorylation on Ser-317 may be crucial for rapid desensitization. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 140 Human
- Entrez Gene: 11542 Mouse
- Entrez Gene: 100911796 Rat
- Entrez Gene: 25370 Rat
- Omim: 600445 Human
- SwissProt: P0DMS8 Human
- SwissProt: P0DMS9 Human
- SwissProt: Q6P2N6 Human
see all -
Alternative names
- A3AR antibody
- A3R antibody
- AA3R antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue sections labeling Adenosine A3 Receptor/A3AR with ab197350 at a 1/20 dilution.
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Anti-Adenosine A3 Receptor/A3AR antibody (ab197350) at 1/100 dilution + mouse testis tissue lysate at 20 µg
Secondary
Anti-rabbit IgG at 1/8000 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
Exposure time: 1 minuteGel: 10%SDS-PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab197350 has been referenced in 2 publications.
- Lillo A et al. Expression of the Adenosine A2A-A3 Receptor Heteromer in Different Brain Regions and Marked Upregulation in the Microglia of the Transgenic APPSw,Ind Alzheimer's Disease Model. Biomedicines 10:N/A (2022). PubMed: 35203424
- Fujimoto K et al. Adenosine Attenuates Aortic Smooth Muscle Cell Calcification through A3 Adenosine Receptor. Tohoku J Exp Med 249:275-283 (2019). PubMed: 31875581