![Western blot - Adenosine Receptor A2a antibody [7FG-G5-A2] (ab79714) image](#Adenosine-Receptor-A2a-Primary-antibodies-ab79714-1.jpg)
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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> More GPCR
Overview
Product name
Anti-Adenosine Receptor A2a antibody [7FG-G5-A2]
See all Adenosine Receptor A2a products (7) ...
Description
Mouse monoclonal [7FG-G5-A2] to Adenosine Receptor A2a
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Guinea pig, Hamster, Dog, Non Human Primates
Immunogen
Full length protein (Human)
Epitope
Recognises amino acids 213-220 (SQPLPGER) within the third intracellular loop.
Positive control
Adenosine Receptor A2a transfected HEK cells
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 0.5mg/ml BSA, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified by running the antiserum from the injected animal through an affinity column with the antigen bound to a beaded agarose gel.
Clonality
Monoclonal
Clone number
7FG-G5-A2
Isotype
IgG2a
Research areas
Neuroscience >> Neurology process >> Neuroregeneration >> Neuroregeneration
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Cell Biology >> Apoptosis >> Phagocytosis
Cardiovascular >> Blood >> Coagulation >> Regulatory
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> More GPCR
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Western blot - Adenosine Receptor A2a antibody [7FG-G5-A2] (ab79714)
![Western blot - Adenosine Receptor A2a antibody [7FG-G5-A2] (ab79714) image](/ps/datasheet/images/79/ab79714/Adenosine-Receptor-A2a-Primary-antibodies-ab79714-1.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab79714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 5 µg/mlPredicted molecular weight: 45 kDa.
Target
Function
Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Domain
The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface.
Post-translational
modifications
Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP29274
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A2AAR antibody
- A2aR antibody
- AA2AR_HUMAN antibody
see all
Database links
- Entrez Gene: 135 Human
- Entrez Gene: 11540 Mouse
- Entrez Gene: 25369 Rat
- Omim: 102776 Human
- SwissProt: P11617 Dog
- SwissProt: P29274 Human
- SwissProt: Q60613 Mouse
- SwissProt: P30543 Rat
see all
Anti-Adenosine Receptor A2a antibody [7FG-G5-A2] images:
Western blot - Adenosine Receptor A2a antibody [7FG-G5-A2] (ab79714)
All lanes : Anti-Adenosine Receptor A2a antibody [7FG-G5-A2] (ab79714) at 5 µg/ml
Lane 1 : Adenosine Receptor A2a transfected HEK cell membrane fraction at 6.5 µg
Lane 2 : Adenosine Receptor A2a transfected HEK cell membrane fraction at 13 µg
Lane 3 : Adenosine Receptor A2a transfected HEK cell membrane fraction at 26 µg
Predicted band size : 45 kDa
Observed band size : 49 kDa (why is the actual band size different from the predicted?)
The difference in the observed and predicted band sizes may be due to glycosylation.
References for Anti-Adenosine Receptor A2a antibody [7FG-G5-A2] (ab79714)
This product has been referenced in:
- Bone DBet al. Nucleoside/nucleobase transport and metabolism by microvascular endothelial cells isolated from ENT1-/- mice. Am J Physiol Heart Circ Physiol 299:H847-56 (2010). WB; Mouse.Read more (PubMed: 20543083) »
See 1 publication for this product
Publishing research using ab79714? Please let us know so that we can cite the reference in this datasheet
![Adenosine Receptor A2a antibody [7FG-G5-A2] for Immunocytochemistry/ Immunofluorescence in Human (79714)](/ps/datasheet/images/79/ab79714/Adenosine-Receptor-A2a-Primary-antibodies-ab79714-6.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"