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Western blot - Adenosine Receptor A2a antibody [7FG-G5-A2] (ab79714)
![Western blot - Adenosine Receptor A2a antibody [7FG-G5-A2] (ab79714)](/ps/datasheet/images/79/ab79714/Adenosine-Receptor-A2a-Primary-antibodies-ab79714-1.jpg)
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Product Name
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Adenosine Receptor A2a antibody [7FG-G5-A2]
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See all Adenosine Receptor A2a antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [7FG-G5-A2] to Adenosine Receptor A2a
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Immunogen
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Full length protein (Human)
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Dog, Gpig, Hm, NHuPrm
(Due to sequence homology)
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Epitope
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Recognises amino acids 213-220 (SQPLPGER) within the third intracellular loop.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 5 µg/ml. Predicted molecular weight: 45 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Adenosine Receptor A2a transfected HEK cells
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Cellular localization
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Cell Membrane; multi pass membrane protein
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Research areas
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Neuroscience >> Neurology process >> Neuroregeneration >> Neuroregeneration
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Cell Biology >> Apoptosis >> Phagocytosis
Cardiovascular >> Blood >> Coagulation >> Regulatory
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> More GPCR
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Relevance
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This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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7FG-G5-A2
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Isotype
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IgG2a
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 0.5mg/ml BSA, pH 7.2 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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Purified by running the antiserum from the injected animal through an affinity column with the antigen bound to a beaded agarose gel.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Adenosine Receptor A2a antibody [7FG-G5-A2] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Adenosine Receptor A2a antibody [7FG-G5-A2] images, references, products related to ab79714 and other tools.
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Adenosine Receptor A2a antibody [7FG-G5-A2] images:
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 Western blot - Adenosine Receptor A2a antibody [7FG-G5-A2] (ab79714)
All lanes : Adenosine Receptor A2a antibody [7FG-G5-A2] (ab79714) at 5 µg/ml
Lane 1 : Adenosine Receptor A2a transfected HEK cell membrane fraction at 6.5 µg
Lane 2 : Adenosine Receptor A2a transfected HEK cell membrane fraction at 13 µg
Lane 3 : Adenosine Receptor A2a transfected HEK cell membrane fraction at 26 µg
Predicted band size : 45 kDa
Observed band size : 49 kDa (why is the actual band size different from the predicted?)
The difference in the observed and predicted band sizes may be due to glycosylation.
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Search PubMed (MEDLINE) for references to Adenosine Receptor A2a
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Adenosine Receptor A2a antibody [7FG-G5-A2] - more information
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Customer reviews (feedback) regarding Adenosine Receptor A2a antibody [7FG-G5-A2] |
Customer FAQs regarding Adenosine Receptor A2a antibody [7FG-G5-A2] |
Protocols for Adenosine Receptor A2a antibody [7FG-G5-A2] |
Price and availability of products related to Adenosine Receptor A2a antibody [7FG-G5-A2] |
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Adenosine Receptor A2a antibody [7FG-G5-A2] - related products:
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Adenosine Receptor A2a antibody [7FG-G5-A2] - other tools:
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Contact Abcam with a Technical Enquiry about Adenosine Receptor A2a antibody [7FG-G5-A2] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - Adenosine Receptor A2a antibody [7FG-G5-A2] (ab79714)](/index.html?datasheet=79714#Adenosine-Receptor-A2a-Primary-antibodies-ab79714-1.jpg)
