Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> More GPCR
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A researcher from Mexico needs to use a Anti-Adenosine Receptor A2a antibody in rat and CoIP. I found ab3461 but it has not been tested for rat. Could you check the homology to see if you would recommend trying this antibody and if the 100% testing discount would apply. He also needs Anti-Dopamine D2 Receptor antibody to use in rat and CoIP. I found ab21218 that has been tested in rat and IP, but he wants to know if it will work in CoIP as well. |
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Hi Daniela, I did an alignment between the rat sequence and the immnunogen sequence for ab3461 and I would not recommend using this antibody in rat. The level of similarity between the two is to low. I would however recommend ab101678, it has not been tested in IP, but it will react with rat tissue. The customer could get a testing discount for trying it out in IP. As for ab21218, I think that one should work in CoIP, if it works in IP, it should work for CoIP. Let me know if there is anything else I can help with. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ICC/IF image of ab3461 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3461, 10µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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